Nature of immobilized antibody layers linked to thioctic acid treated gold surfaces

Utilization of 125I-labeled IgG enables an investigation of protein immobilized to gold electrodes sputter deposited on microporous nylon membranes, including the precise nature of the surface–protein bond (i.e. covalent or non-specific adsorption), physical location of the immobilized protein (i.e....

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Bibliographic Details
Published in:Biosensors & bioelectronics 2000-06, Vol.15 (3), p.183-192
Main Authors: Smith, Aaron M, Ducey, Michael W, Meyerhoff, Mark E
Format: Article
Language:English
Subjects:
125I-labeled protein
Adsorption
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biotechnology
Electrochemistry
Fundamental and applied biological sciences. Psychology
Gold
Immobilization of enzymes and other molecules
Immobilization techniques
Immunoassay - instrumentation
Immunoassay - methods
Immunoglobulin G
Indicators and Reagents
Iodine Radioisotopes
Methods. Procedures. Technologies
Microporous gold electrodes
Non-specific adsorption
Nylon membranes
Polystyrenes
Protein immobilization
Thioctic Acid
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Summary:Utilization of 125I-labeled IgG enables an investigation of protein immobilized to gold electrodes sputter deposited on microporous nylon membranes, including the precise nature of the surface–protein bond (i.e. covalent or non-specific adsorption), physical location of the immobilized protein (i.e. on the surface of the gold electrode or within the pores of the membrane), and the amount of protein immobilized. This is accomplished by comparing the mass of protein immobilized to gold surfaces that have been treated in several different fashions, as well as, deposition of the gold on nylon membranes that have been treated differently. It is shown that these microporous gold electrodes, proposed previously for conducting novel non-separation electrochemical enzyme immunoassays, consist of multiple protein layers non-specifically adsorbed. Approximately, half of the total adsorbed protein is immobilized to the gold surface with the remaining protein bound within the pores on the nylon membrane.
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(00)00062-2