Growth of enteric neurones from isolated myenteric ganglia in dissociated cell culture

Ganglia of the myenteric plexus from the newborn guinea-pig, isolated by microdissection, were dissociated by a combination of enzymatic and mechanical methods. The neurones and glial cells in the resulting cell suspension were cultured for up to 21 days in vitro. The growth of the enteric ganglion...

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Bibliographic Details
Published in:Cell and tissue research 1991-09, Vol.265 (3), p.527-534
Main Authors: SAFFREY, M. J, BAILEY, D. J, BURNSTOCK, G
Format: Article
Language:English
Subjects:
Animal cells
Animals
Biological and medical sciences
Cecum - innervation
Cell cultures. Hybridization. Fusion
Cell Division
Cells, Cultured
Enkephalin, Methionine - analysis
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Ganglia, Autonomic - cytology
Guinea Pigs
Immunohistochemistry
Microscopy, Phase-Contrast
Molecular and cellular biology
Myenteric Plexus - cytology
Neuroglia - cytology
Neurons - cytology
Neuropeptides - analysis
Substance P - analysis
Ubiquitin Thiolesterase
Vasoactive Intestinal Peptide - analysis
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Summary:Ganglia of the myenteric plexus from the newborn guinea-pig, isolated by microdissection, were dissociated by a combination of enzymatic and mechanical methods. The neurones and glial cells in the resulting cell suspension were cultured for up to 21 days in vitro. The growth of the enteric ganglion cells in serum-free, hormone-supplemented (N1) medium and in serum-supplemented medium containing a mitotic inhibitor was compared over a period of 14 days in vitro. Enteric neurones were outnumbered by glia in both culture media, although glial cell proliferation was inhibited in both media compared with that in serum-supplemented medium without mitotic inhibitors. Glial cell numbers appeared to decline in serum-free medium after the first week in vitro. Neurites tended to be more varicose in the serum-free medium, and the morphology of the enteric glial cells also differed markedly in the two media. This is the first report of the dissociation and subsequent culture of myenteric ganglia that had previously been completely isolated from the remainder of the gut wall.
ISSN:0302-766X
1432-0878
DOI:10.1007/BF00340876