Stereospecific high-performance liquid chromatographic assay of sotalol in plasma

A convenient high-performance liquid chromatographic (HPLC) assay was developed for determination of sotalol (STL) enantiomers in plasma. Following addition of the internal standard (IS; racemic atenolol), enantiomers of STL and IS were extracted using ethyl acetate. After evaporation of the organic...

Full description

Saved in:
Bibliographic Details
Published in:Pharmaceutical research 1991-09, Vol.8 (9), p.1195-1198
Main Authors: CARR, R. A, FOSTER, R. T, BHANJI, N. H
Format: Article
Language:English
Subjects:
Adult
Analysis
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Cyanates - blood
General pharmacology
Humans
Isocyanates
Male
Medical sciences
Naphthalenes - blood
Pharmacology. Drug treatments
Sotalol - blood
Stereoisomerism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 1198
container_issue 9
container_start_page 1195
container_title Pharmaceutical research
container_volume 8
creator CARR, R. A
FOSTER, R. T
BHANJI, N. H
description A convenient high-performance liquid chromatographic (HPLC) assay was developed for determination of sotalol (STL) enantiomers in plasma. Following addition of the internal standard (IS; racemic atenolol), enantiomers of STL and IS were extracted using ethyl acetate. After evaporation of the organic layer, samples were derivatized with a solution of S-(+)-1-(1-naphthyl)ethyl isocyanate (NEIC). The resulting diastereomers were chromatographed with normal-phase HPLC with chloroform:hexane:methanol [65:33:2 (v/v)] as the mobile phase at a flow rate of 2 ml/min. The fluorescence detection wavelength was set at 220 nm for excitation with no emission filter. The suitability of the assay for pharmacokinetic studies was determined by measuring STL enantiomers in the plasma of a healthy subject after administration of a single 160-mg oral, racemic dose of STL.
doi_str_mv 10.1023/A:1015870805757
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_72655932</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72655932</sourcerecordid><originalsourceid>FETCH-LOGICAL-p167t-7029e1a63688cb7d74a3e65597a3efa12befc3a7474435ed974c0aff6e9196963</originalsourceid><addsrcrecordid>eNo9kE1LxDAYhIMo67p69iT0IN6qSfPVeFsWv2BBRAVv5d002UbSTTdpD_vvrVg8zWEeZphB6JLgW4ILere8J5jwUuISc8nlEZoTLmmuMPs6RnMsC5aXkpFTdJbSN8a4JIrN0IzIsiRCztHbe2-iCakz2lmns8Ztm7wz0YbYwk6bzLv94OpMNzG00IdthK4ZOUgJDlmwWQo9-OAzt8s6D6mFc3RiwSdzMekCfT4-fKye8_Xr08tquc67sbjPJS6UISCoKEu9kbVkQI3gXMlRLZBiY6ymIJlkjHJTK8k0BmuFUUQJJegC3fzldjHsB5P6qnVJG-9hZ8KQKln8ptFiBK8mcNi0pq666FqIh2r6YPSvJx-SBm_juNulf4xjKjih9AciS2uH</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72655932</pqid></control><display><type>article</type><title>Stereospecific high-performance liquid chromatographic assay of sotalol in plasma</title><source>SpringerLink_过刊（NSTL购买）</source><creator>CARR, R. A ; FOSTER, R. T ; BHANJI, N. H</creator><creatorcontrib>CARR, R. A ; FOSTER, R. T ; BHANJI, N. H</creatorcontrib><description>A convenient high-performance liquid chromatographic (HPLC) assay was developed for determination of sotalol (STL) enantiomers in plasma. Following addition of the internal standard (IS; racemic atenolol), enantiomers of STL and IS were extracted using ethyl acetate. After evaporation of the organic layer, samples were derivatized with a solution of S-(+)-1-(1-naphthyl)ethyl isocyanate (NEIC). The resulting diastereomers were chromatographed with normal-phase HPLC with chloroform:hexane:methanol [65:33:2 (v/v)] as the mobile phase at a flow rate of 2 ml/min. The fluorescence detection wavelength was set at 220 nm for excitation with no emission filter. The suitability of the assay for pharmacokinetic studies was determined by measuring STL enantiomers in the plasma of a healthy subject after administration of a single 160-mg oral, racemic dose of STL.</description><identifier>ISSN: 0724-8741</identifier><identifier>EISSN: 1573-904X</identifier><identifier>DOI: 10.1023/A:1015870805757</identifier><identifier>PMID: 1788167</identifier><identifier>CODEN: PHREEB</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Adult ; Analysis ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Cyanates - blood ; General pharmacology ; Humans ; Isocyanates ; Male ; Medical sciences ; Naphthalenes - blood ; Pharmacology. Drug treatments ; Sotalol - blood ; Stereoisomerism</subject><ispartof>Pharmaceutical research, 1991-09, Vol.8 (9), p.1195-1198</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=5036513$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1788167$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CARR, R. A</creatorcontrib><creatorcontrib>FOSTER, R. T</creatorcontrib><creatorcontrib>BHANJI, N. H</creatorcontrib><title>Stereospecific high-performance liquid chromatographic assay of sotalol in plasma</title><title>Pharmaceutical research</title><addtitle>Pharm Res</addtitle><description>A convenient high-performance liquid chromatographic (HPLC) assay was developed for determination of sotalol (STL) enantiomers in plasma. Following addition of the internal standard (IS; racemic atenolol), enantiomers of STL and IS were extracted using ethyl acetate. After evaporation of the organic layer, samples were derivatized with a solution of S-(+)-1-(1-naphthyl)ethyl isocyanate (NEIC). The resulting diastereomers were chromatographed with normal-phase HPLC with chloroform:hexane:methanol [65:33:2 (v/v)] as the mobile phase at a flow rate of 2 ml/min. The fluorescence detection wavelength was set at 220 nm for excitation with no emission filter. The suitability of the assay for pharmacokinetic studies was determined by measuring STL enantiomers in the plasma of a healthy subject after administration of a single 160-mg oral, racemic dose of STL.</description><subject>Adult</subject><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Cyanates - blood</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Isocyanates</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Naphthalenes - blood</subject><subject>Pharmacology. Drug treatments</subject><subject>Sotalol - blood</subject><subject>Stereoisomerism</subject><issn>0724-8741</issn><issn>1573-904X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNo9kE1LxDAYhIMo67p69iT0IN6qSfPVeFsWv2BBRAVv5d002UbSTTdpD_vvrVg8zWEeZphB6JLgW4ILere8J5jwUuISc8nlEZoTLmmuMPs6RnMsC5aXkpFTdJbSN8a4JIrN0IzIsiRCztHbe2-iCakz2lmns8Ztm7wz0YbYwk6bzLv94OpMNzG00IdthK4ZOUgJDlmwWQo9-OAzt8s6D6mFc3RiwSdzMekCfT4-fKye8_Xr08tquc67sbjPJS6UISCoKEu9kbVkQI3gXMlRLZBiY6ymIJlkjHJTK8k0BmuFUUQJJegC3fzldjHsB5P6qnVJG-9hZ8KQKln8ptFiBK8mcNi0pq666FqIh2r6YPSvJx-SBm_juNulf4xjKjih9AciS2uH</recordid><startdate>19910901</startdate><enddate>19910901</enddate><creator>CARR, R. A</creator><creator>FOSTER, R. T</creator><creator>BHANJI, N. H</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19910901</creationdate><title>Stereospecific high-performance liquid chromatographic assay of sotalol in plasma</title><author>CARR, R. A ; FOSTER, R. T ; BHANJI, N. H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p167t-7029e1a63688cb7d74a3e65597a3efa12befc3a7474435ed974c0aff6e9196963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adult</topic><topic>Analysis</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Cyanates - blood</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Isocyanates</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Naphthalenes - blood</topic><topic>Pharmacology. Drug treatments</topic><topic>Sotalol - blood</topic><topic>Stereoisomerism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CARR, R. A</creatorcontrib><creatorcontrib>FOSTER, R. T</creatorcontrib><creatorcontrib>BHANJI, N. H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CARR, R. A</au><au>FOSTER, R. T</au><au>BHANJI, N. H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stereospecific high-performance liquid chromatographic assay of sotalol in plasma</atitle><jtitle>Pharmaceutical research</jtitle><addtitle>Pharm Res</addtitle><date>1991-09-01</date><risdate>1991</risdate><volume>8</volume><issue>9</issue><spage>1195</spage><epage>1198</epage><pages>1195-1198</pages><issn>0724-8741</issn><eissn>1573-904X</eissn><coden>PHREEB</coden><abstract>A convenient high-performance liquid chromatographic (HPLC) assay was developed for determination of sotalol (STL) enantiomers in plasma. Following addition of the internal standard (IS; racemic atenolol), enantiomers of STL and IS were extracted using ethyl acetate. After evaporation of the organic layer, samples were derivatized with a solution of S-(+)-1-(1-naphthyl)ethyl isocyanate (NEIC). The resulting diastereomers were chromatographed with normal-phase HPLC with chloroform:hexane:methanol [65:33:2 (v/v)] as the mobile phase at a flow rate of 2 ml/min. The fluorescence detection wavelength was set at 220 nm for excitation with no emission filter. The suitability of the assay for pharmacokinetic studies was determined by measuring STL enantiomers in the plasma of a healthy subject after administration of a single 160-mg oral, racemic dose of STL.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>1788167</pmid><doi>10.1023/A:1015870805757</doi><tpages>4</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0724-8741
ispartof Pharmaceutical research, 1991-09, Vol.8 (9), p.1195-1198
issn 0724-8741
1573-904X
language eng
recordid cdi_proquest_miscellaneous_72655932
source SpringerLink_过刊（NSTL购买）
subjects Adult
Analysis
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Cyanates - blood
General pharmacology
Humans
Isocyanates
Male
Medical sciences
Naphthalenes - blood
Pharmacology. Drug treatments
Sotalol - blood
Stereoisomerism
title Stereospecific high-performance liquid chromatographic assay of sotalol in plasma
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T11%3A00%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Stereospecific%20high-performance%20liquid%20chromatographic%20assay%20of%20sotalol%20in%20plasma&rft.jtitle=Pharmaceutical%20research&rft.au=CARR,%20R.%20A&rft.date=1991-09-01&rft.volume=8&rft.issue=9&rft.spage=1195&rft.epage=1198&rft.pages=1195-1198&rft.issn=0724-8741&rft.eissn=1573-904X&rft.coden=PHREEB&rft_id=info:doi/10.1023/A:1015870805757&rft_dat=%3Cproquest_pubme%3E72655932%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-p167t-7029e1a63688cb7d74a3e65597a3efa12befc3a7474435ed974c0aff6e9196963%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=72655932&rft_id=info:pmid/1788167&rfr_iscdi=true