RNA editing of sorghum mitochondrial atp6 transcripts changes 15 amino acids and generates a carboxy-terminus identical to yeast

• Published in: Current genetics 1991-11, Vol.20 (5), p.417-422
• Main Authors: Kempken, F. (Florida Univ., Gainesville (USA). Dept. of Plant Pathology and United States Dept. of Agriculture/Agricultural Research Service), Mullen, J.A, Pring, D.R, Tang, H.V
• Format: Article
• Language: English
• Subjects: ACIDE AMINE; ADENOSINE TRIPHOSPHATE; ADENOSINTRIFOSFATO; AMINO ACIDS; AMINOACIDOS; ARN; atp6; Base Sequence; Biological and medical sciences; Cloning, Molecular; DNA, Mitochondrial; Edible Grain; Fundamental and applied biological sciences. Psychology; Genetic Variation; MITOCHONDRIA; Mitochondria - metabolism; MITOCHONDRIE; MITOCONDRIA; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Proton-Translocating ATPases - genetics; Proton-Translocating ATPases - metabolism; RNA; RNA Processing, Post-Transcriptional; Saccharomyces cerevisiae - genetics; Sequence Homology, Nucleic Acid; SORGHUM BICOLOR; Sorghum mitochondria; Transcript editing; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/bf00317071

Sequencing of sorghum mitochondrial atp6 cDNA clones revealed 19 C-to-U transcript editing events within a 756 bp-conserved core gene; three were silent and 16 resulted in 15 amino acid changes. Only one edit, which was silent, was found in the 381 bp amino-extension to the core gene. Eleven of the 15 changed amino acids were identical with or else represented conservative changes compared to yeast atp6. Editing of a CAA codon to TAA truncates the carboxy-terminus to a position identical to that of yeast. The frequency of editing at sites which change amino acids was very high in contrast to partial editing at silent, third base, sites.