Effects of Adrenomedullin on Human Myocyte Contractile Function and β-Adrenergic Response

• Published in: Journal of cardiovascular pharmacology and therapeutics 2002-10, Vol.7 (4), p.235-240
• Main Authors: Mukherjee, Rupak, Multani, Marlina M., Sample, Jeffrey A., Dowdy, Kathryn B., Zellner, James L., Hoover, Donald B., Spinale, Francis G.
• Format: Article
• Language: English
• Subjects: Adrenomedullin; Aged; Analysis of Variance; Depression, Chemical; Humans; Middle Aged; Myocardial Contraction - drug effects; Myocardial Contraction - physiology; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - physiology; Peptides - pharmacology; Receptors, Adrenergic, beta - physiology
• ISSN: 1074-2484; 1940-4034
• DOI: 10.1177/107424840200700406

Background: Adrenomedullin has been demonstrated to cause systemic vasodilation, and increased plasma adrenomedullin levels have been observed in cardiovascular disease states such as heart failure. While adrenomedullin receptors have been localized to the myocardium, the effects of adrenomedullin on human myocyte contractility remained unknown. Methods and Results: Left ventricular myocytes were isolated from myocardial biopsies of patients (n = 16) undergoing elective coronary artery bypass surgery with normal left ventricular ejection fractions (51 ± 1%). A total of 233 left ventricular myocytes were studied by videomicroscopy. Myocyte shortening velocity (,um/s) was measured at baseline and following the addition of either 3 nM, 30 nM, or 60 nM of adrenomedullin. The change in myocyte shortening velocity with increasing concentrations of adrenomedullin was computed. At all concentrations, adrenomedullin reduced myocyte shortening velocity from baseline values (P < 0.05). Next, the potential interaction of adrenomedullin with the P-adrenergic receptor system was examined using 25 nM isoproterenol. The 3-adrenergic receptor-mediated increase in the myocyte shortening velocity was blunted with adrenomedullin (29 ± 7 vs 63 ± 13 tm/s, P < 0.05). Conclusions: These unique findings demonstrate that adrenomedullin reduced contractility in isolated human left ventricular myocytes and exhibited a negative interaction with the fadrenergic receptor system. Past studies have shown that adrenomedullin induces nitric oxide synthesis and that nitric oxide can uncouple myocyte metabolism. Thus, while adrenomedullin causes systemic vasodilation, this peptide can also exert a negative contractile effect in human left ventricular myocytes.