A Single Mutation Affects Both N-Acetylglucosaminyltransferase and Glucuronosyltransferase Activities in a Chinese Hamster Ovary Cell Mutant Defective in Heparan Sulfate Biosynthesis

Mutants of Chinese hamster ovary cells have been found that no longer produce heparan sulfate. Characterization of one of the mutants, pgsD-677, showed that it lacks both N-acetylglucosaminyl- and glucuronosyltransferase, enzymes required for the polymerization of heparan sulfate chains. pgsD-677 al...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1992-03, Vol.89 (6), p.2267-2271
Main Authors: Lidholt, Kerstin, Weinke, Julie L., Kiser, Cheryl S., Lugemwa, Fulgentius N., Bame, Karen J., Cheifetz, Sela, Massague, Joan, Lindahl, Ulf, Esko, Jeffrey D.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Autoradiography
Biochemistry
Biological and medical sciences
Biosynthesis
Carbohydrates
Cell Fusion
Cells
Cellular biology
CHO Cells
Chromatography, High Pressure Liquid
Cricetinae
Enzymes
Fundamental and applied biological sciences. Psychology
Genetic mutation
Genetics
Glucosyltransferases - genetics
Glucuronosyltransferase - genetics
Glycosaminoglycans
Glycosaminoglycans - biosynthesis
Glycosaminoglycans - isolation & purification
Heparitin Sulfate - biosynthesis
Holosides
Hybridity
Medical research
Mutation
N-Acetylglucosaminyltransferases
Other biological molecules
Phenotype
Proteoglycans
Rodents
Sulfates
Sulfates - metabolism
Sulfur Radioisotopes
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Summary:Mutants of Chinese hamster ovary cells have been found that no longer produce heparan sulfate. Characterization of one of the mutants, pgsD-677, showed that it lacks both N-acetylglucosaminyl- and glucuronosyltransferase, enzymes required for the polymerization of heparan sulfate chains. pgsD-677 also accumulates 3- to 4-fold more chondroitin sulfate than the wild type. Cell hybrids derived from pgsD-677 and wild type regained both transferase activities and the capacity to synthesize heparan sulfate. Two segregants from one of the hybrids reexpressed the dual enzyme deficiency, the lack of heparan sulfate synthesis, and the enhanced accumulation of chondroitin sulfate, suggesting that all of the traits were genetically linked. These findings indicate that the pgsD locus may represent a gene involved in the coordinate control of glycosaminoglycan formation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.89.6.2267