Electric potential control of DNA immobilization on gold electrode

The assembly of synthetic, controllable molecules is one of the goals in nanotechnology. The primary objective of this contribution is to selectively immobilize DNA on gold via electric potential control. The self-assembly monolayer (SAM) was prepared with 2-aminoethanethiol (AET) on the gold electr...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2003, Vol.18 (1), p.53-58
Main Authors: Ge, Cunwang, Liao, Jianhui, Yu, Wei, Gu, Ning
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques
Biotechnology
Buffers
Controlled potential
DNA - analysis
DNA - chemistry
DNA immobilize
DNA-based device
Electrodes
Fundamental and applied biological sciences. Psychology
Gold
Immobilization of enzymes and other molecules
Immobilization techniques
Methods. Procedures. Technologies
Nanotechnology
Static Electricity
Time Factors
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Summary:The assembly of synthetic, controllable molecules is one of the goals in nanotechnology. The primary objective of this contribution is to selectively immobilize DNA on gold via electric potential control. The self-assembly monolayer (SAM) was prepared with 2-aminoethanethiol (AET) on the gold electrode. A new approach based on electric potential was firstly used to control DNA immobilization covalently onto the SAM with the activation of 1-ethyl-3(3-dimethyl-aminopropyl)-carbodiimide (EDC) and N-hydroxysulfosuccinimide (NHS) in low ionic strength solution. The influence of electric potential on DNA immobilization was investigated by means of cyclic voltammogram, A.C. impedance, auger electron spectrometer as well as atomic force microscope (AFM) on template-stripped gold surface. The result proves that controlled potential can affect the course of DNA immobilization. More negative potential can restrain the DNA immobilization, while the more positive potential can accelerate the DNA immobilization. It is of great significance for the control of DNA self-assembly and will find wide application in the fields of DNA-based devices.
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(02)00115-X