Characterization and chromosomal distribution of a tandemly repeated DNA sequence from the Australian sheep blowfly, Lucilia cuprina

In the course of making a Lucilia cuprina genomic DNA library, a ladder of bands was seen in partial Sau3A digests. Complete digestion reduced this ladder to predominantly monomer units of approximately 190 bp. Nine independently isolated copies of this repeat were cloned and sequenced. Only two of...

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Bibliographic Details
Published in:Chromosoma 1992-03, Vol.101 (5/6), p.358-364
Main Authors: Perkins, H.D, Bedo, D.G, Howells, A.J
Format: Article
Language:English
Subjects:
Animals
autosomes
Base Sequence
Biological and medical sciences
Blotting, Southern
centromeres
Chromosomes
Cloning, Molecular
Diptera - genetics
DNA
embl.x57588
embl/x57584
embl/x57585
embl/x57586
embl/x57587
embl/x57589
embl/x57590
embl/x57591
Fundamental and applied biological sciences. Psychology
Genes. Genome
Lucilia cuprina
Mitosis
mitotic chromosomes
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nucleic Acid Hybridization
nucleotide sequences
polytene chromosomes
repetitive sequences
Repetitive Sequences, Nucleic Acid
satellite DNA
Sequence Homology, Nucleic Acid
sex chromosomes
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Summary:In the course of making a Lucilia cuprina genomic DNA library, a ladder of bands was seen in partial Sau3A digests. Complete digestion reduced this ladder to predominantly monomer units of approximately 190 bp. Nine independently isolated copies of this repeat were cloned and sequenced. Only two of these isolates are identical in sequence, the most divergent being 71% homologous. This satellite DNA occurs in all three wild-type strains tested, and, for the single case examined, in the embryonic, larval, pupal, and adult DNA. It represents approximately 3%-4% of the genome. Data obtained from in situ chromosome hybridizations indicate that this sequence is concentrated around the centromeric regions of the autosomes and over most of the sex chromosomes. Labelling is much stronger in mitotic compared with polytene chromosomes showing directly that this centromeric satellite DNA is grossly under-replicated during polytenization. This under-replication is even more pronounced on the sex chromosomes compared with the autosomes.
ISSN:0009-5915
1432-0886
DOI:10.1007/BF00346015