In vitro binding of hepatitis C virus to CD81-positive and -negative human cell lines

Aim: The aim of the present study is to study the mechanism of entry of hepatitis C virus (HCV) into human cells. We examined the in vitro binding of HCV to various human cell lines with or without CD81 expression. Methods: We first used three cell lines, two hepatocyte‐derived, huH‐7 and HepG2, and...

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Published in:Journal of gastroenterology and hepatology 2003-01, Vol.18 (1), p.74-79
Main Authors: SASAKI, MINA, YAMAUCHI, KATSUMI, NAKANISHI, TOSHIMI, KAMOGAWA, YUMIKO, HAYASHI, NAOAKI
Format: Article
Language:English
Subjects:
Antibodies - pharmacology
Antigens, CD - genetics
Antigens, CD - immunology
Antigens, CD - metabolism
Biological and medical sciences
CD81
Cell Line
Disease Susceptibility
Hepacivirus - genetics
Hepacivirus - physiology
hepatitis C virus
Hepatocytes - chemistry
Human viral diseases
Humans
in vitro binding
Infectious diseases
Medical sciences
Membrane Proteins - genetics
Membrane Proteins - immunology
Membrane Proteins - metabolism
reverse transcriptase-polymerase chain reaction
RNA, Viral - analysis
Tetraspanin 28
Transfection
Viral diseases
Viral hepatitis
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Summary:Aim: The aim of the present study is to study the mechanism of entry of hepatitis C virus (HCV) into human cells. We examined the in vitro binding of HCV to various human cell lines with or without CD81 expression. Methods: We first used three cell lines, two hepatocyte‐derived, huH‐7 and HepG2, and one colon cancer cell line, Cw2. Among them, HepG2 did not express TAPA‐1 (CD81) on their surface but two others did. They were incubated with HCV + serum for 1 h and HCV‐RNA in extracted RNA obtained from these cells was examined by using both a quantitative test and reverse transcriptase–polymerase chain reaction (RT‐PCR). Results: We found that a significant amount of HCV‐RNA was detected in huH‐7 and HepG2 but not in Cw2. In addition, the titer of HCV‐RNA in serum‐incubated CD81‐transfected HepG2 was similar to that of the non‐transfected titer, and the binding between HCV and huH‐7 was not inhibited by anti‐CD81. Under the same condition, HCV‐RNA tended to be detectable in serum‐pulsed hepatocyte‐derived cell lines, but not in the others. Conclusion: These results suggest that while CD81, as reported, specifically binds to HCV‐E2 protein, the entry of HCV into human hepatocytes might be regulated by CD81‐unrelated molecule(s). © 2003 Blackwell Publishing Asia Pty Ltd
ISSN:0815-9319
1440-1746
DOI:10.1046/j.1440-1746.2003.02925.x