Ischemia/reperfusion injury of skeletal muscle: Plasma taurine as a measure of tissue damage

Background. Cell membrane rupture by oxygen-derived free radicals is a systematic feature of ischemia/reperfusion (I/R) injury. High taurine concentration gradients in skeletal muscle prompted us to evaluate whether plasma taurine levels (pTau) are a useful marker of I/R injury after different perio...

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Bibliographic Details
Published in:Surgery 2003-01, Vol.133 (1), p.91-100
Main Authors: Nanobashvili, Joseph, Neumayer, Christoph, Fügl, Alexander, Punz, Andreas, Blumer, Roland, Prager, Manfred, Mittlböck, Martina, Gruber, Helmut, Polterauer, Peter, Roth, Erich, Malinski, Tadeusz, Huk, Ihor
Format: Article
Language:English
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Animals
Biological and medical sciences
Biomarkers
Cell Membrane - pathology
Creatine Kinase - blood
Emergency and intensive postoperative care (general aspects). Pathophysiology of surgery
Intensive care medicine
L-Lactate Dehydrogenase - blood
Male
Malondialdehyde - analysis
Medical sciences
Microscopy, Electron
Muscle Contraction
Muscle, Skeletal - metabolism
Muscle, Skeletal - pathology
Muscle, Skeletal - ultrastructure
Rabbits
Recovery of Function
Reperfusion Injury - blood
Reperfusion Injury - pathology
Taurine - analysis
Taurine - blood
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Summary:Background. Cell membrane rupture by oxygen-derived free radicals is a systematic feature of ischemia/reperfusion (I/R) injury. High taurine concentration gradients in skeletal muscle prompted us to evaluate whether plasma taurine levels (pTau) are a useful marker of I/R injury after different periods of ischemia. Methods. Rabbits were randomly assigned to either 1 or 2.5 hours of hind-limb ischemia followed by 2 hours of reperfusion (groups IR1 [n = 12] and IR2.5 [n = 13], respectively). Corresponding sham groups (SHAM1 [n = 8] and SHAM2.5 [n = 9]) were used as controls. Analyzed parameters included histomorphometry and electron microscopy of skeletal muscle biopsies, pTau, and plasma level of malondialdehyde. Skeletal muscle function was assessed 3 weeks after I/R injury. Results. No significant morphologic changes were detectable at the end of ischemia. After reperfusion, mild interstitial edema with intact muscle cell membranes developed in IR1 group; pTau was not increased. IR2.5 group, by contrast, showed severe interstitial edema formation (interfiber area increased by 112%, P
ISSN:0039-6060
1532-7361
DOI:10.1067/msy.2003.65