Sphingosylphosphorylcholine stimulates cellular fibronectin expression through upregulation of IL-6 in cultured human dermal fibroblasts

Sphingosylphosphorylcholine (SPC) has been reported to stimulate wound healing by its potent mitogenic effect. Fibronectin (FN) is a cell-adhesion protein that plays an important role in cell migration and collagen deposition during wound healing. In order to elucidate further the mechanism involved...

Full description

Saved in:
Bibliographic Details
Published in:Archives of Dermatological Research 2003, Vol.294 (10-11), p.433-437
Main Authors: SUHR, Ki-Beom, TSUBOI, Ryoji, SEO, Eun-Young, PIAO, Yong-Jun, LEE, Jeung-Hoon, PARK, Jang-Kyu, OGAWA, Hideoki
Format: Article
Language:English
Subjects:
Antibodies - pharmacology
Biological and medical sciences
Cells, Cultured
Dermatology
Dermis - cytology
Dermis - metabolism
Fibroblasts - metabolism
Fibronectins - genetics
Fibronectins - metabolism
Humans
Interleukin-6 - immunology
Interleukin-6 - metabolism
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Phosphorylcholine - analogs & derivatives
Phosphorylcholine - pharmacology
Protein Biosynthesis
RNA, Messenger - antagonists & inhibitors
RNA, Messenger - metabolism
Sphingosine - analogs & derivatives
Sphingosine - pharmacology
Transcription, Genetic
Up-Regulation
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sphingosylphosphorylcholine (SPC) has been reported to stimulate wound healing by its potent mitogenic effect. Fibronectin (FN) is a cell-adhesion protein that plays an important role in cell migration and collagen deposition during wound healing. In order to elucidate further the mechanism involved in the accelerated wound healing stimulated by SPC, we studied the role of SPC in FN production in cultured human dermal fibroblasts. We demonstrated that SPC dose- and time-dependently enhanced the expression of FN in human dermal fibroblast at the protein and mRNA levels. IL-6 is known to stimulate the production of FN in fibroblasts. SPC also markedly induced IL-6 production in cultured human dermal fibroblasts in a dose- and time-dependent manner. We also demonstrated that FN mRNA expression in human dermal fibroblasts was upregulated 4 h after IL-6 treatment. Moreover, pretreatment with neutralizing anti-IL-6 antibodies partially blocked the upregulation of FN mRNA expression induced by SPC in human dermal fibroblasts. These results indicate that SPC may stimulate FN synthesis through IL-6 production in cultured human dermal fibroblasts.
ISSN:0340-3696
1432-069X
DOI:10.1007/s00403-002-0357-4