Methods for measuring the infrared spectra of biological cells

Infrared (IR) spectroscopy of biological cells is a growing area of research, with many papers focusing on differences between the spectra of cancerous and noncancerous cells. Much of this research has been performed using a monolayer of dehydrated cells. We posit that the use of monolayers can intr...

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Bibliographic Details
Published in:Physics in medicine & biology 2003-01, Vol.48 (2), p.243-257
Main Authors: Mourant, Judith R, Gibson, Rowena R, Johnson, Tamara M, Carpenter, Susan, Short, Kurt W, Yamada, Yujiro R, Freyer, James P
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carcinoma - chemistry
Cell Line
Desiccation
DNA - chemistry
Fibroblasts - chemistry
Investigative techniques, diagnostic techniques (general aspects)
Lipids - chemistry
Male
Medical sciences
Miscellaneous. Technology
Neoplasms, Glandular and Epithelial - chemistry
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Prostatic Neoplasms - chemistry
Proteins - chemistry
Rats
Rats, Inbred F344
RNA - chemistry
Sensitivity and Specificity
Spectrophotometry, Infrared - methods
Spectroscopy, Fourier Transform Infrared - methods
Spectrum Analysis, Raman - methods
Tumor Cells, Cultured - chemistry
Water - chemistry
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Summary:Infrared (IR) spectroscopy of biological cells is a growing area of research, with many papers focusing on differences between the spectra of cancerous and noncancerous cells. Much of this research has been performed using a monolayer of dehydrated cells. We posit that the use of monolayers can introduce artefacts that lead to an apparent but inaccurate measurement of differences between cancerous and noncancerous cells. Additionally, the use of dried cells complicates the extraction of biochemical information from the IR spectra. We demonstrate that using suspensions of viable cells in aqueous suspension reduces measurement artefacts and facilitates determining the concentration of the major biochemical components via a linear least-squares fit of the component spectra to the spectrum of the cells.
ISSN:0031-9155
1361-6560
DOI:10.1088/0031-9155/48/2/307