Cytotoxic T-cell activity is not detectable in Venezuelan equine encephalitis virus-infected mice

Previously published research has established that the immune response to the Venezuelan equine encephalitis virus (VEEV) vaccine strain TC-83 is Th 1-mediated, with local activation of both CD4+ and CD8+ T cells. This suggests that cytotoxic lymphocytes CTL may play a role in protection against vir...

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Bibliographic Details
Published in:Virus research 2003-02, Vol.91 (2), p.255-259
Main Authors: Jones, L.D, Bennett, A.M, Moss, S.R, Gould, E.A, Phillpotts, R.J
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Cytotoxic lymphocytes
Encephalitis Virus, Venezuelan Equine - immunology
Encephalitis Virus, Venezuelan Equine - pathogenicity
Encephalomyelitis, Venezuelan Equine - immunology
Encephalomyelitis, Venezuelan Equine - prevention & control
Haplotypes
Immunity
Immunization
Lymph Nodes - cytology
Lymph Nodes - immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred CBA
Spleen - cytology
Spleen - immunology
T-Lymphocytes, Cytotoxic - immunology
Vaccinia virus - genetics
Venezuelan equine encephalitis
Viral Proteins - genetics
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
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Summary:Previously published research has established that the immune response to the Venezuelan equine encephalitis virus (VEEV) vaccine strain TC-83 is Th 1-mediated, with local activation of both CD4+ and CD8+ T cells. This suggests that cytotoxic lymphocytes CTL may play a role in protection against virulent VEEV.? Studies involving a variety of immunisation schedules with either TC-83 or strain CAAR 508 (serogroup 5) of VEEV, and six different haplotypes of mice, failed to reveal functional CTL activity against VEEV-infected targets in secondary antigen-stimulated lymphocyte cultures from either the draining lymph nodes (LN) or spleen. Nor were VEEV-specific CTL detected after immunisation of mice (three haplotypes) with recombinant vaccinia viruses (VV) expressing either the non-structural (nsP1-4) or the structural (C-E3-E2-6K-E1) genes of TC-83. Reciprocal experiments in which mice were immunised with TC-83, and their lymphocytes tested against VV recombinant-infected targets also failed to detect CTL activity. These data suggest that VEEV infection of mice does not elicit detectable CTL activity, and that CTL are unlikely to play a role in protection against virulent VEEV.
ISSN:0168-1702
1872-7492
DOI:10.1016/S0168-1702(02)00275-7