Ethanolamine plasmalogen and cholesterol reduce the total membrane oxidizability measured by the oxygen uptake method

To investigate the effects of ethanolamine plasmalogen, phosphatidylethanolamine, cholesterol, and α-tocopherol on the oxidizability of membranes, various large unilamellar vesicles (LUVs) including these lipids and antioxidant were examined for their total membrane oxidizabilities, evaluated as R p...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-03, Vol.302 (2), p.265-270
Main Authors: Maeba, Ryouta, Ueta, Nobuo
Format: Article
Language:English
Subjects:
alpha-Tocopherol - pharmacology
Animals
Cattle
Cell Membrane - drug effects
Cell Membrane - metabolism
Cholesterol
Cholesterol - pharmacology
Drug Combinations
Ethanolamine plasmalogen
Free Radical Scavengers
Free radicals
Membranes
Oxidation
Oxidation-Reduction - drug effects
Oxidizability
Oxygen - metabolism
Oxygen consumption
Phosphatidylethanolamines - pharmacology
Phospholipids - metabolism
Plasmalogens
Plasmalogens - pharmacology
Radical scavenger
Vesicles
Vinyl ether double bond
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Summary:To investigate the effects of ethanolamine plasmalogen, phosphatidylethanolamine, cholesterol, and α-tocopherol on the oxidizability of membranes, various large unilamellar vesicles (LUVs) including these lipids and antioxidant were examined for their total membrane oxidizabilities, evaluated as R p/ R i 1/2 value (where R p is rate of oxygen consumption and R i 1/2 is the square root of rate of chain initiation) by the oxygen uptake method with water-soluble radical initiator and inhibitor. Incorporation of bovine brain ethanolamine plasmalogen (BBEP) into vesicles as well as cholesterol led to lower the total membrane oxidizability dose-dependently. The effect of BBEP was more efficient in the presence of cholesterol in vesicles. On the other hand, diacyl counterpart, egg yolk phosphatidylethanolamine, and a typical radical scavenger, α-tocopherol, had no effect on the membrane oxidizability. α-Tocopherol only prolonged an induction period dose-dependently in the present oxidizing system, suggesting a novel antioxidant mechanism of ethanolamine plasmalogens besides the action of scavenging radicals.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(03)00157-8