Osteoclasts from medullary bone of egg-laying Japanese quail do not express the putative calcium 'receptor'

The present study reports the contrasting effects of extracellular calcium ([Ca2+]e) elevation on cytosolic free calcium levels ([Ca2+]i) of osteoclasts, freshly isolated either from medullary bone of the egg-laying Japanese quail or from rat cortical bone. [Ca2+]i was measured in single osteoclasts...

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Bibliographic Details
Published in:Experimental physiology 1992-05, Vol.77 (3), p.501-504
Main Authors: Bascal, ZA, Moonga, BS, Dacke, CG, Zaidi, M
Format: Article
Language:English
Subjects:
Animals
Bone and Bones - cytology
Calcium - analysis
Calcium - metabolism
Coturnix
Coturnix japonica
Extracellular Matrix - chemistry
Female
Ionomycin - pharmacology
Male
Osteoclasts - chemistry
Osteoclasts - ultrastructure
Rats
Rats, Inbred Strains
Receptors, Cell Surface - analysis
Receptors, Cell Surface - metabolism
Spectrometry, Fluorescence
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Summary:The present study reports the contrasting effects of extracellular calcium ([Ca2+]e) elevation on cytosolic free calcium levels ([Ca2+]i) of osteoclasts, freshly isolated either from medullary bone of the egg-laying Japanese quail or from rat cortical bone. [Ca2+]i was measured in single osteoclasts using the Ca(2+)-sensitive fluorochrome, Indo-1. We found that elevation of [Ca2+]e failed to induce a rise of [Ca2+]i in quail osteoclasts, whilst causing an elevation of [Ca2+]i in rat osteoclasts. The calcium ionophore, ionomycin, led to a sustained elevation of [Ca2+]i in both cell types. These findings suggest that osteoclasts isolated from egg-laying quail do not possess the calcium sensor or 'receptor' that appears to be vital for the survival and function of rat osteoclasts.
ISSN:0958-0670
1469-445X
DOI:10.1113/expphysiol.1992.sp003610