Molecular characterization of the novel rat NK receptor 1C7

• Published in: European journal of immunology 2003-02, Vol.33 (2), p.342-351
• Main Authors: Bäckman‐Petersson, Eva, Miller, J. Ross, Hollyoake, Martine, Aguado, Begoña, Butcher, Geoffrey W.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Cell Line; Cloning; Cytotoxicity; Cytotoxicity, Immunologic; DNA, Complementary - genetics; Genes; Haplorhini; Humans; Immunoglobulin Fc Fragments - genetics; Killer Cells, Natural - chemistry; Ligands; Mice; Molecular Sequence Data; Multigene Family; Natural Cytotoxicity Triggering Receptor 3; NK receptor; Polymorphism, Genetic; Protein Structure, Tertiary; Rat; Rats - genetics; Rats, Inbred Strains; Receptors, Immunologic - biosynthesis; Receptors, Immunologic - chemistry; Receptors, Immunologic - genetics; Receptors, Immunologic - physiology; Recombinant Fusion Proteins - physiology; Sequence Alignment; Sequence Homology, Amino Acid; Species Specificity; Specific Pathogen-Free Organisms; T-Lymphocytes - chemistry; Transfection; Tumor
• ISSN: 0014-2980; 1521-4141
• DOI: 10.1002/immu.200310008

A novel receptor, named 1C7 or NKp30 and involved in natural cytotoxicity, was recently identified. This receptor is encoded by the 1C7 gene, which is located within the class III region of the human MHC, HLA. It is a member of the immunoglobulin gene superfamily (IgSF) and, remarkably, is expressed at the mRNA level as six different splice variants in human. Recent investigations have indicated that the 1c7 gene of the mouse is silenced by in‐frame stop codons. In this study, the molecular characterization of the rat 1c7 gene is described. cDNA derived from this gene encode a protein of 192 amino acid residues predicted to contain a single IgV‐set domain in the extracellular region and a positively charged residue in the transmembrane region. Expression of the gene was detected in freshly isolated rat Natural Killer (NK) and T splenocytes. Transfection of rat 1C7 into the NK cell line RNK‐16 induced cytolytic activity against glioma as well as lymphoma tumor cells. In addition, binding of a r1C7‐Fc fusion protein by a panel of target cells correlated with susceptibility to killing by RNK‐16–1C7 effector cells. These results indicate that the r1C7 molecule could function as an NK activating receptor as previously reported for the human NKp30 receptor molecule.