Comparison of glutamine synthetases from brains of genetically epilepsy prone and genetically epilepsy resistant rats

Since glutamine synthetase (GS) has been proposed as the primary enzyme in the regulation of glutamate metabolism in the central nervous system and since inhibition of the activity of this enzyme in vivo leads to seizures, it has been proposed that an abnormality in the structure or function of this...

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Bibliographic Details
Published in:Neurochemical research 1992-10, Vol.17 (10), p.1015-1019
Main Authors: Carl, G F, Thompson, L A, Williams, J T, Wallace, V C, Gallagher, B B
Format: Article
Language:English
Subjects:
Animals
Brain - enzymology
Disease Models, Animal
Disease Susceptibility
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Epilepsy - enzymology
Glutamate-Ammonia Ligase - chemistry
Immunity, Innate
Isoelectric Focusing
Molecular Weight
Rats
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Summary:Since glutamine synthetase (GS) has been proposed as the primary enzyme in the regulation of glutamate metabolism in the central nervous system and since inhibition of the activity of this enzyme in vivo leads to seizures, it has been proposed that an abnormality in the structure or function of this enzyme could be responsible for the induction of seizures in epilepsy prone rats. To test this hypothesis the glutamine synthetases were purified from the brains of both genetically epilepsy prone rats (GEPR) and their progenitors, genetically epilepsy resistant rats (GERR). The enzymes were compared using both SDS-PAGE and isoelectric focusing. The immunoreactivities of equal amounts of protein were determined using the ELISA technique, and the regulation of the glutamine synthetase activities by Mn2+/Mg2+ ratios were compared. The only difference found between the glutamine synthetases from the two strains was a slightly lower specific activity of the enzyme from the epilepsy prone animals.
ISSN:0364-3190
1573-6903
DOI:10.1007/bf00966830