Concentration-dependent activities of the even-skipped protein in Drosophila embryos

The Drosophila pair-rule gene even-skipped (eve) encodes a homeo-domain-containing protein (Eve) that is required for the development of both odd- and even-numbered parasegments. We have used a heat shock-inducible eve transgene to study the regulatory functions of Eve in vivo. Transcripts encoded b...

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Bibliographic Details
Published in:Genes & development 1992-09, Vol.6 (9), p.1740-1751
Main Authors: MANOUKIAN, A. S, KRAUSE, H. M
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins
Biological and medical sciences
DNA-Binding Proteins - physiology
Drosophila
Drosophila - embryology
Drosophila - genetics
Drosophila Proteins
Fundamental and applied biological sciences. Psychology
Fushi Tarazu Transcription Factors
Gene expression
Gene Expression Regulation - genetics
Genes, Homeobox - genetics
Homeodomain Proteins
Insect Hormones - genetics
Molecular and cellular biology
Molecular genetics
Mutation - genetics
Phenotype
Repressor Proteins - physiology
Transcription Factors
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Summary:The Drosophila pair-rule gene even-skipped (eve) encodes a homeo-domain-containing protein (Eve) that is required for the development of both odd- and even-numbered parasegments. We have used a heat shock-inducible eve transgene to study the regulatory functions of Eve in vivo. Transcripts encoded by eight other segmentation genes were monitored for changes in distribution and abundance following short pulses of ectopic Eve expression. Two tiers of response times appeared to distinguish between genes that were direct [fushi tarazu (ftz), odd-skipped (odd), runt (run), paired, and wingless] and indirect [eve, hairy, and engrailed (en)] targets of Eve. Genes that appeared to be directly regulated by Eve were differentially repressed in a concentration-dependent fashion. Interestingly, the run and ftz genes could also be activated by Eve during a brief 20- to 30-min stage in development. The delayed actions upon the eve and en genes appeared to be mediated by run and odd. As in eve- embryos, these effects on segmentation gene expression patterns caused defects in both odd- and even-numbered parasegments. Four sequential phenotypes could be induced, each of which was attributable to the altered expression of a unique subset of target genes.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.6.9.1740