Electroconvulsive shock increases serotonin transporter in the rat frontal cortex

The antidepressive action of electroconvulsive shock (ECS) is thought to involve the alteration in serotonin (5-HT) neurotransmission, including the increase in 5-HT release and uptake. In our previous study, 5-HT transporter (5-HTT) mRNA expression was decreased after single and repetitive ECS in r...

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Bibliographic Details
Published in:Neuroscience letters 2003-05, Vol.341 (2), p.170-172
Main Authors: Shen, Hao-wei, Numachi, Yohtaro, Yoshida, Sumiko, Fujiyama, Ko, Toda, Shigenobu, Awata, Shuichi, Matsuoka, Hiroo, Sato, Mitsumoto
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blotting, Western
Carrier Proteins - genetics
Carrier Proteins - metabolism
DNA Primers
Electroconvulsive shock
Electroconvulsive therapy
Electroshock
Frontal cortex
Frontal Lobe - metabolism
Gene Expression Regulation
Hippocampus
Hippocampus - metabolism
Male
Medical sciences
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Membrane Transport Proteins
Nerve Tissue Proteins
Psychology. Psychoanalysis. Psychiatry
Psychopathology. Psychiatry
Raphe Nuclei - metabolism
Raphe nucleus
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction - methods
Serotonin Plasma Membrane Transport Proteins
Serotonin transporter
Time Factors
Treatments
Western blotting
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Summary:The antidepressive action of electroconvulsive shock (ECS) is thought to involve the alteration in serotonin (5-HT) neurotransmission, including the increase in 5-HT release and uptake. In our previous study, 5-HT transporter (5-HTT) mRNA expression was decreased after single and repetitive ECS in rat raphe nucleus. In the present study, we investigated the effects of single and repetitive ECS on the protein levels of 5-HTT in the frontal cortex, hippocampus and raphe nucleus of rat brain using quantitative Western blot analysis. Single ECS did not alter 5-HTT protein expression in any brain regions examined. Repetitive ECS stably increased 5-HTT protein in the frontal cortex, but not in the hippocampus and raphe nucleus. Because ECS is known to facilitate the release of neurotransmitters, our results suggest that the increased 5-HTT protein expression in the frontal cortex might be a compensatory change against the enhanced 5-HT release by ECS in presynaptic terminals.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(03)00178-2