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A role for the interleukin-1 receptor in the pathway linking static mechanical compression to decreased proteoglycan synthesis in surface articular cartilage
Loading of articular cartilage during weight bearing is essential for the maintenance of cartilage function. Although certain cyclic loading protocols stimulate extracellular matrix synthesis, constant or static compression decreases proteoglycan and collagen synthesis in cartilage explants. The goa...
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Published in: | Archives of biochemistry and biophysics 2003-05, Vol.413 (2), p.229-235 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Loading of articular cartilage during weight bearing is essential for the maintenance of cartilage function. Although certain cyclic loading protocols stimulate extracellular matrix synthesis, constant or static compression decreases proteoglycan and collagen synthesis in cartilage explants. The goal of this study was to determine whether the compression-induced decrease in proteoglycan synthesis involves an interleukin-1 (IL-1) signaling pathway. Cartilage explants were compressed 50% in the presence of IL-1 receptor antagonist (IL-1ra), and the incorporation of [
35
S
]sulfate into macromolecules was measured. IL-1ra increased sulfate incorporation in compressed cartilage but not in cartilage maintained at the in situ thickness (0% compression). IL-1α and IL-1β mRNAs were detected in cartilage compressed 50% for at least 3
h, while nitric oxide synthase II mRNA was only detected in cartilage compressed 50% for 6
h. The data support a role for the IL-1 receptor in the pathway linking static compression to reduced proteoglycan synthesis. |
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ISSN: | 0003-9861 1096-0384 |
DOI: | 10.1016/S0003-9861(03)00129-2 |