Role of dietary copper in enhancing resistance to Escherichia coli mastitis

• Published in: Journal of dairy science 2003-04, Vol.86 (4), p.1240-1249
• Main Authors: Scaletti, R.W, Trammell, D.S, Smith, B.A, Harmon, R.J
• Format: Article
• Language: English
• Subjects: animal diseases; animal pathogenic bacteria; Animal productions; Animals; Biological and medical sciences; blood chemistry; body temperature; Body Weight; bovine mastitis; Cattle; cattle diseases; Cell Count; Ceruloplasmin - analysis; copper; Copper - administration & dosage; Copper - analysis; Copper - blood; dairy cows; Diet; dietary mineral supplements; Dietary Supplements; disease course; disease resistance; dry matter intake; duration; Eating; Escherichia coli; Escherichia coli Infections - prevention & control; Escherichia coli Infections - veterinary; Female; ferroxidase; Fundamental and applied biological sciences. Psychology; heifers; infection; Iron - blood; Kinetics; Lactation; liver; Liver - chemistry; Mastitis, Bovine - prevention & control; Milk - cytology; Milk - microbiology; Minerals - analysis; Minerals - blood; plate count; postpartum period; Pregnancy; prepartum period; selenium; Selenium - blood; somatic cell count; Terrestrial animal productions; Vertebrates; zinc; Zinc - blood
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.s0022-0302(03)73708-4

The role of dietary copper in enhancing resistance to Escherichia coli mastitis was investigated in first-lactation heifers. Twenty-three primigravid Holstein heifers were maintained on a basal (6.5 ppm copper; -Cu) diet or a diet supplemented (20 ppm) with copper sulfate (+Cu) beginning 60 d prepartum through 42 d of lactation. Liver biopsies and blood samples were taken for liver and blood minerals and plasma ceruloplasmin. Milk samples were taken weekly postpartum for bacteriology. The overall mean liver Cu concentration was about threefold higher, and the overall mean plasma Cu concentration was greater in the +Cu group than the -Cu group. At 34 d of lactation, one pathogen-free quarter per animal was infused with 22 cfu of Escherichia coli strain 727. Plasma Cu was greater at -24, 0, 18, 24, 36, 96, 192, and 240 h relative to infusion for +Cu animals. Plasma Zn concentration was higher at 24 h for the +Cu group. Milk bacterial count (log10 cfu/ml) was lower at 12, 18, and 48 h for the +Cu group. Somatic cell count (log10/ml) was lower at 18 h in +Cu animals. Clinical score at 24 h was lower for +Cu cows, while at 144 h, clinical score was lower for -Cu cows. Rectal temperature was lower at 18 h for the +Cu group. Plasma ceruloplasmin and Fe, dry matter intake and milk production did not differ. Copper supplementation reduced the clinical response during experimental E. coli mastitis, but duration was unchanged.