Highly sensitive quantification of vancomycin in plasma samples using liquid chromatography–tandem mass spectrometry and oral bioavailability in rats

We developed a highly sensitive liquid chromatography–tandem mass spectrometry assay (LC–MS–MS) for a glycopeptide antibacterial drug, vancomycin (VCM), in rat plasma. After precipitating 100 μl of plasma with 300 μl of 10% trifluoroacetic acid–methanol (2:1, v/v), the supernatant was diluted with 3...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-06, Vol.789 (2), p.211-218
Main Authors: Shibata, Nobuhito, Ishida, Makoto, Venkata Rama Prasad, Yarasani, Gao, Weihua, Yoshikawa, Yukako, Takada, Kanji
Format: Article
Language:English
Subjects:
Administration, Oral
Analysis
Animals
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - blood
Anti-Bacterial Agents - pharmacokinetics
Biological and medical sciences
Biological Availability
Calibration
Chromatography, Liquid - methods
General pharmacology
Male
Mass Spectrometry - methods
Medical sciences
Pharmacology. Drug treatments
Rats
Rats, Wistar
Reproducibility of Results
Sensitivity and Specificity
Vancomycin
Vancomycin - administration & dosage
Vancomycin - blood
Vancomycin - pharmacokinetics
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Summary:We developed a highly sensitive liquid chromatography–tandem mass spectrometry assay (LC–MS–MS) for a glycopeptide antibacterial drug, vancomycin (VCM), in rat plasma. After precipitating 100 μl of plasma with 300 μl of 10% trifluoroacetic acid–methanol (2:1, v/v), the supernatant was diluted with 300 μl of distilled water and was passed through a filter. LC–MS–MS equipped with electrospray ionization in the positive ion mode used a pair of ions at 725/144 m/ z for VCM in the multiple reaction-monitoring mode with a sample injection volume of 20 μl. The calibration curve had a linear range from 0.01 to 20 μg/ml when linear least square regression was applied to the concentration versus peak area plot. The drug in the sample was detected within 5 min. Precision, accuracy and limit of quantitation indicated that this method was suitable for the quantitative determination of VCM in rat plasma. Using this method, we defined for the first time that the oral bioavailability of VCM in rats was 0.069%. This method can be applied to basic pharmacokinetic and pharmaceutical studies in rats.
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(03)00068-0