Expression, Purification, Physicochemical Characterization and Structural Analysis of Cytochrome c554 from Vibrio parahaemolyticus Strain RIMD2210633

The function of cytochrome c(554) of Vibrio parahaemolyticus has not yet been determined. We have determined the physicochemical properties and crystal structure of cytochrome c(554) at 1.8 A in order to help elucidate its function. The physicochemical properties and the tertiary structure of cytoch...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2010, Vol.74 (5), p.1113-1115
Main Authors: AKAZAKI, Hideharu, KAWAI, Fumihiro, CHIDA, Hirotaka, HIRANO, Takako, HAKAMATA, Wataru, PARK, Sam-Yong, NISHIO, Toshiyuki, OKU, Tadatake
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical Phenomena
Crystallography, X-Ray
Cytochrome c Group - chemistry
Cytochrome c Group - genetics
Cytochrome c Group - isolation & purification
Cytochrome c Group - metabolism
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Gene Expression
Models, Molecular
Oxidation-Reduction
Protein Structure, Secondary
Protein Structure, Tertiary
Pseudomonas - enzymology
Spectrophotometry, Ultraviolet
Vibrio parahaemolyticus - enzymology
Vibrio parahaemolyticus - genetics
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Summary:The function of cytochrome c(554) of Vibrio parahaemolyticus has not yet been determined. We have determined the physicochemical properties and crystal structure of cytochrome c(554) at 1.8 A in order to help elucidate its function. The physicochemical properties and the tertiary structure of cytochrome c(554) resemble those of dimeric cytochrome c(552) from Pseudomonas nautica, but the Vibrio genus contains no gene for nitrite reductase, cytochrome cd(1), in its genome DNA. These results raise the possibility that both cytochromes denote an electron to an electron carrier and accept an electron from same electron carrier.
ISSN:0916-8451
1347-6947