Expression of advanced glycation end-product receptors in the cochlea

Objectives/Hypothesis: Advanced glycation end products (AGE) have recently been implicated in aging changes within different tissues of the body. The role of AGEs and their receptors in the mammalian inner ear is largely unknown. In this study we analyzed for the expression of two AGE receptors, nam...

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Published in:The Laryngoscope 2010-06, Vol.120 (6), p.1227-1232
Main Authors: Hanusek, Claudia, Setz, Cristian, Radojevic, Vesna, Brand, Yves, Levano, Soledad, Bodmer, Daniel
Format: Article
Language:English
Subjects:
Advanced glycation end product receptors
Animals
Biological and medical sciences
Blotting, Western
Cochlea - chemistry
Ddost (AGE-R1)
Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology
hair cells
Immunohistochemistry
inner ear
Medical sciences
Mice
Mice, Inbred C57BL
Non tumoral diseases
Otorhinolaryngology. Stomatology
presbycusis
RAGE
Rats
Receptor for Advanced Glycation End Products
Receptors, Immunologic - metabolism
Reverse Transcriptase Polymerase Chain Reaction
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Summary:Objectives/Hypothesis: Advanced glycation end products (AGE) have recently been implicated in aging changes within different tissues of the body. The role of AGEs and their receptors in the mammalian inner ear is largely unknown. In this study we analyzed for the expression of two AGE receptors, namely RAGE and Ddost (AGE‐R1). Study Design: Controlled animal study. Controlled animal study. This study confirmed the expression of the AGEs receptors RAGE and Ddost (AGE‐R1) both on mRNA and protein level in the cochlea. Furthermore, we were able to localize these two receptors in the organ of Corti (OC). Methods: Expression of RAGE and Ddost (AGE‐R1) receptors in the rat cochlea were analyzed by reverse transcriptase‐polymerase chain reaction (RT‐PCR) and Western blot. Specific localization of these two AGE receptors was also obtained within the mouse OC using immunohistochemistry. Results: We detected RAGE and Ddost (AGE‐R1) at the mRNA and protein level in the OC, spiral ganglion, and stria vascularis. Moreover, RAGE and Ddost (AGE‐R1) could specifically be identified within the immature and mature OC. Conclusions: The AGE receptors RAGE and Ddost (AGE‐R1) could be identified within the cochlea. Different expression patterns of these two receptors were observed in the immature and mature inner ear. Laryngoscope, 120:1227–1232, 2010
ISSN:0023-852X
1531-4995
DOI:10.1002/lary.20940