Implantation of Capillary Structure Engineered by Optical Lithography Improves Hind Limb Ischemia in Mice

We previously reported a novel optical lithographic technique for the construction of a capillary network consisting of endothelial cells. To investigate the feasibility of clinical application in the treatment of ischemic diseases, capillary structures were formed on scaffolds made from amniotic me...

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Bibliographic Details
Published in:Tissue engineering. Part A 2010-03, Vol.16 (3), p.953-959
Main Authors: Akahori, Taichi, Kobayashi, Akiko, Komaki, Motohiro, Hattori, Hideshi, Nakahama, Ken-Ichi, Ichinose, Shizuko, Abe, Mayumi, Takeda, Satoru, Morita, Ikuo
Format: Article
Language:English
Subjects:
Amnion - metabolism
Animals
Blood Vessel Prosthesis
Capillaries
Capillaries - pathology
Care and treatment
Cattle
Hindlimb - blood supply
Hindlimb - pathology
Hindlimb - physiopathology
Humans
Ischemia
Ischemia - pathology
Ischemia - physiopathology
Mice
Mice, Inbred BALB C
Neovascularization, Physiologic
Optical Phenomena
Optics
Original Articles
Photolithography
Physiological aspects
Prosthesis Implantation - methods
Regional Blood Flow
Rodents
Tissue engineering
Tissue Engineering - methods
Transplants & implants
Veins & arteries
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Summary:We previously reported a novel optical lithographic technique for the construction of a capillary network consisting of endothelial cells. To investigate the feasibility of clinical application in the treatment of ischemic diseases, capillary structures were formed on scaffolds made from amniotic membrane (AM) and implanted into mice. The capillary network remained in place for at least 5 days and blood perfusion through the implanted capillaries was histologically detected in an ear flap model. Moreover, blood was observed flowing through the capillary network implanted in abdominal subcutaneous tissue of mice at 5 days after insertion. Implantation of the AM capillary structure into the ischemic hind limbs of mice significantly increased reperfusion compared with controls (AM only). Blood flow was restored in the ischemic limbs to the level of corresponding nonischemic limbs as early as 9 days after surgical implantation. The treatment reversed ischemic symptoms, and ambulatory impairment was significantly improved. Thus, the implantation of a capillary network engineered ex vivo could have therapeutic potential for ischemic diseases.
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2009.0097