Use of microbial electrodes for observation of microbiological nitrogen elimination process

Microbial electrodes for the determination of ammonia and the estimation of biochemical oxygen demand (BOD) were applied to the nitrogen elimination process. The dimensions of the nitrification and the denitrification vessels were 170 and 70 L, respectively. The wastewater used for the experiment wa...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1992-06, Vol.40 (1), p.130-136
Main Authors: Hikuma, M. (Nishi-Tokyo University, Yamanashi, Japan), Matsuoka, H, Kawarai, M, Karube, I
Format: Article
Language:English
Subjects:
ammonia
biochemical oxygen demand
Biological and medical sciences
Biosensors
Biotechnology
CONSOMMATION D'OXYGENE
CONSUMO DE OXIGENO
DENITRIFICACION
DENITRIFICATION
ELECTRODE
ELECTRODOS
Fundamental and applied biological sciences. Psychology
IMMOBILISATION
INMOVILIZACION
MEDICION
MESURE
Methods. Procedures. Technologies
microbial electrode
Microbiological nitrogen elimination process
NITRIFICACION
NITRIFICATION
nitrifying bacteria
TRAITEMENT DES EAUX USEES
TRATAMIENTO DE AGUAS RESIDUALES
TRICHOSPORON
Trichosporon cutaneum
Various methods and equipments
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Summary:Microbial electrodes for the determination of ammonia and the estimation of biochemical oxygen demand (BOD) were applied to the nitrogen elimination process. The dimensions of the nitrification and the denitrification vessels were 170 and 70 L, respectively. The wastewater used for the experiment was obtained from a fermentation factory and adjusted to 470-530 mg/L of total Kjeldahl nitrogen and 1700-3000 mg/L of BOD. The ammonia electrode was assembled with a membrane containing nitrifying bacteria and an oxygen probe. The BOD electrode was similarly constructed, except it used a membrane containing the yeast, Trichosporon cutaneum. A flow system was employed for the automatic measurement of samples every 30 min. The nitrification and denitrification rates of the activated sludge were measured to investigate the optimum conditions and evaluate the capacity of the plant. The various data obtained by the microbial electrodes allowed us to inspect the situation of the plant and estimate control parameters such as nitrogen and BOD loadings. The average removals of ammonia nitrogen and total Kjeldahl nitrogen were 96% and 89%, respectively, during the experiment for period of 2 weeks
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260400118