Differential histone acetylation in alfalfa (Medicago sativa) due to growth in NaCl: responses in salt stressed and salt tolerant callus cultures

The steady state distribution of histone variant proteins and their modifications by acetylation were characterized in wild type and salinity stress adapted alfalfa (Medicago sativa). Isotopic labeling detected dynamic acetylation at four sites in the histone H3 variants and five sites in histones H...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1989-05, Vol.90 (1), p.237-245
Main Authors: Waterborg, J.H. (University of Missouri Kansas City, Kansas City, MO), Harrington, R.E, Winicov, I
Format: Article
Language:English
Subjects:
Acetylation
Alfalfa
CAL
CALLO
Callus
Cell lines
CHLORURE DE SODIUM
Chromatin
CLORURO DE SODIO
CRECIMIENTO
CROISSANCE
CULTIVO DE TEJIDOS
CULTURE DE TISSUS
Gels
HISTONAS
HISTONE
Histones
MEDICAGO SATIVA
Salt tolerance
Salts
STRESS
Table salt
TOLERANCE AU SEL
TOLERANCIA A LA SAL
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Summary:The steady state distribution of histone variant proteins and their modifications by acetylation were characterized in wild type and salinity stress adapted alfalfa (Medicago sativa). Isotopic labeling detected dynamic acetylation at four sites in the histone H3 variants and five sites in histones H4 and H2B. Histone variant H3.2 was the most highly acetylated histone with 25% higher steady state acetylation and a two- to threefold higher acetylation labeling than histone H3.1. Histone phosphorylation was limited to histone variants H1.A, H1.B, and H1.C and to histone H2A.3, which was also acetylated. Histone variant composition was unaffected by cellular exposure to NaCl. Histone acetylation was qualitatively similar in salt-tolerant and salt-sensitive cells under normal growth conditions. However, short-term salt stress in salt sensitive cells or continued growth at 1% NaCl in salt tolerant cells led to major increases in the multiacetylated forms of histone H4 and the two variants of histone H3. These changes were more pronounced in the diploid than in the tetraploid alfalfa strains. The increase in multiacetylation of core histones serves as an in vivo reporter suggesting an altered intranuclear ionic environment in the presence of salt. It may also represent an adaptive response in chromatin structure to permit chromatin function in a more saline intranuclear environment
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.90.1.237