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Urease-null and hydrogenase-null phenotypes of a phylloplane bacterium reveal altered nickel metabolism in two soybean mutants
Mutation at either of two genetic loci (Eu2 or Eu3) in soybean (Glycine max [L.] Merr.) results in a pleiotropic elimination of the activity of both major urease isozymes. Surprisingly, the phenotype of a phylloplane bacterium, Methylobacterium mesophilicum, living on the leaves of eu2/eu2 or eu3-e1...
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Published in: | Plant physiology (Bethesda) 1992-03, Vol.98 (3), p.942-948 |
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creator | Holland, M.A. (University of Missouri, Columbia, MO) Polacco, J.C |
description | Mutation at either of two genetic loci (Eu2 or Eu3) in soybean (Glycine max [L.] Merr.) results in a pleiotropic elimination of the activity of both major urease isozymes. Surprisingly, the phenotype of a phylloplane bacterium, Methylobacterium mesophilicum, living on the leaves of eu2/eu2 or eu3-e1/eu3-e1 mutants is also affected by these plant mutations. The bacteria isolated from leaves of these soybean mutants have transient urease- and hydrogenase-deficient phenotypes that can be corrected by the addition of nickel to free-living cultures. The same bacterium growing on wild-type soybeans or on urease mutants eu1-sun/ eu1-sun or eu4/eu4, each deficient in only one urease isozyme, are urease-positive. These results suggest that the bacterium living on the eu2/eu2 or eu3-e1/eu3-e1 mutant is unable to produce an active urease or hydrogenase because it is effectively starved for nickel. We infer that mutations at Eu2 or Eu3 result in defects in nickel metabolism but not in Ni(2+) uptake or transport, because eu2/eu2 and eu3-e1/eu3-e1 mutants exhibit normal uptake of 63NiCl2. Moreover, wild-type plants grafted on mutant rootstocks produce seeds with fully active urease, indicating unimpeded transport of nickel through mutant roots and stems |
doi_str_mv | 10.1104/pp.98.3.942 |
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Surprisingly, the phenotype of a phylloplane bacterium, Methylobacterium mesophilicum, living on the leaves of eu2/eu2 or eu3-e1/eu3-e1 mutants is also affected by these plant mutations. The bacteria isolated from leaves of these soybean mutants have transient urease- and hydrogenase-deficient phenotypes that can be corrected by the addition of nickel to free-living cultures. The same bacterium growing on wild-type soybeans or on urease mutants eu1-sun/ eu1-sun or eu4/eu4, each deficient in only one urease isozyme, are urease-positive. These results suggest that the bacterium living on the eu2/eu2 or eu3-e1/eu3-e1 mutant is unable to produce an active urease or hydrogenase because it is effectively starved for nickel. We infer that mutations at Eu2 or Eu3 result in defects in nickel metabolism but not in Ni(2+) uptake or transport, because eu2/eu2 and eu3-e1/eu3-e1 mutants exhibit normal uptake of 63NiCl2. Moreover, wild-type plants grafted on mutant rootstocks produce seeds with fully active urease, indicating unimpeded transport of nickel through mutant roots and stems</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.98.3.942</identifier><identifier>PMID: 16668768</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; BACTERIA ; Bacteriology ; Biological and medical sciences ; Callus ; FENOTIPOS ; FEUILLE ; Fundamental and applied biological sciences. Psychology ; Genetic mutation ; Genotypes ; GLYCINE MAX ; HOJAS ; Leaves ; Metabolism. Enzymes ; METABOLISME ; METABOLISMO ; Microbiology ; MUTANT ; MUTANTES ; NICKEL ; NIQUEL ; OXIDORREDUCTASAS ; OXYDOREDUCTASE ; PHENOTYPE ; Phenotypes ; Plants ; Scions ; Seedlings ; Soybeans ; UREASA ; UREASE</subject><ispartof>Plant physiology (Bethesda), 1992-03, Vol.98 (3), p.942-948</ispartof><rights>Copyright 1992 American Society of Plant Physiologists</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3422-8657729f756e884a6eb72f01e701f5ca97b2e92ced88031b92069cd93679c82b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4274191$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4274191$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5443178$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16668768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Holland, M.A. (University of Missouri, Columbia, MO)</creatorcontrib><creatorcontrib>Polacco, J.C</creatorcontrib><title>Urease-null and hydrogenase-null phenotypes of a phylloplane bacterium reveal altered nickel metabolism in two soybean mutants</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Mutation at either of two genetic loci (Eu2 or Eu3) in soybean (Glycine max [L.] Merr.) results in a pleiotropic elimination of the activity of both major urease isozymes. Surprisingly, the phenotype of a phylloplane bacterium, Methylobacterium mesophilicum, living on the leaves of eu2/eu2 or eu3-e1/eu3-e1 mutants is also affected by these plant mutations. The bacteria isolated from leaves of these soybean mutants have transient urease- and hydrogenase-deficient phenotypes that can be corrected by the addition of nickel to free-living cultures. The same bacterium growing on wild-type soybeans or on urease mutants eu1-sun/ eu1-sun or eu4/eu4, each deficient in only one urease isozyme, are urease-positive. These results suggest that the bacterium living on the eu2/eu2 or eu3-e1/eu3-e1 mutant is unable to produce an active urease or hydrogenase because it is effectively starved for nickel. We infer that mutations at Eu2 or Eu3 result in defects in nickel metabolism but not in Ni(2+) uptake or transport, because eu2/eu2 and eu3-e1/eu3-e1 mutants exhibit normal uptake of 63NiCl2. Moreover, wild-type plants grafted on mutant rootstocks produce seeds with fully active urease, indicating unimpeded transport of nickel through mutant roots and stems</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>BACTERIA</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Callus</subject><subject>FENOTIPOS</subject><subject>FEUILLE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic mutation</subject><subject>Genotypes</subject><subject>GLYCINE MAX</subject><subject>HOJAS</subject><subject>Leaves</subject><subject>Metabolism. Enzymes</subject><subject>METABOLISME</subject><subject>METABOLISMO</subject><subject>Microbiology</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>NICKEL</subject><subject>NIQUEL</subject><subject>OXIDORREDUCTASAS</subject><subject>OXYDOREDUCTASE</subject><subject>PHENOTYPE</subject><subject>Phenotypes</subject><subject>Plants</subject><subject>Scions</subject><subject>Seedlings</subject><subject>Soybeans</subject><subject>UREASA</subject><subject>UREASE</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNpFkEuLFDEUhYMoTs_oyp2IZCG4kG7zqjyWMjgqDLjQXodU6tZMjamkTKpGauNvN0037eo-zsfh3oPQK0p2lBLxcZp2Ru_4zgj2BG1ow9mWNUI_RRtCak-0NhfospQHQgjlVDxHF1RKqZXUG_R3n8EV2MYlBOxih-_XLqc7iOfldA8xzesEBaceuzqvIaQpuAi4dX6GPCwjzvAIrjqEOkOH4-B_QcAjzK5NYSgjHiKe_yRc0tqCi3hcZhfn8gI9610o8PJUr9D-5vPP66_b2-9fvl1_ut16LhjbatkoxUyvGglaCyehVawnFBShfeOdUS0Dwzx0WhNOW8OINL4zXCrjNWv5FXp_9J1y-r1Ame04FA_h8EVailWcC6MElZX8cCR9TqVk6O2Uh9Hl1VJiD3nbabJGW25r3pV-e_Jd2hG6_-wp4Aq8OwGueBf67KIfyplrhOBUHbA3R-yhzCmfZcHqTYZW-fVR7l2y7i5Xh_0PQ7Xm9fB_ZFqbbw</recordid><startdate>199203</startdate><enddate>199203</enddate><creator>Holland, M.A. (University of Missouri, Columbia, MO)</creator><creator>Polacco, J.C</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199203</creationdate><title>Urease-null and hydrogenase-null phenotypes of a phylloplane bacterium reveal altered nickel metabolism in two soybean mutants</title><author>Holland, M.A. (University of Missouri, Columbia, MO) ; Polacco, J.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3422-8657729f756e884a6eb72f01e701f5ca97b2e92ced88031b92069cd93679c82b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>BACTERIA</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Callus</topic><topic>FENOTIPOS</topic><topic>FEUILLE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic mutation</topic><topic>Genotypes</topic><topic>GLYCINE MAX</topic><topic>HOJAS</topic><topic>Leaves</topic><topic>Metabolism. Enzymes</topic><topic>METABOLISME</topic><topic>METABOLISMO</topic><topic>Microbiology</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>NICKEL</topic><topic>NIQUEL</topic><topic>OXIDORREDUCTASAS</topic><topic>OXYDOREDUCTASE</topic><topic>PHENOTYPE</topic><topic>Phenotypes</topic><topic>Plants</topic><topic>Scions</topic><topic>Seedlings</topic><topic>Soybeans</topic><topic>UREASA</topic><topic>UREASE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Holland, M.A. (University of Missouri, Columbia, MO)</creatorcontrib><creatorcontrib>Polacco, J.C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holland, M.A. (University of Missouri, Columbia, MO)</au><au>Polacco, J.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Urease-null and hydrogenase-null phenotypes of a phylloplane bacterium reveal altered nickel metabolism in two soybean mutants</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1992-03</date><risdate>1992</risdate><volume>98</volume><issue>3</issue><spage>942</spage><epage>948</epage><pages>942-948</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Mutation at either of two genetic loci (Eu2 or Eu3) in soybean (Glycine max [L.] Merr.) results in a pleiotropic elimination of the activity of both major urease isozymes. Surprisingly, the phenotype of a phylloplane bacterium, Methylobacterium mesophilicum, living on the leaves of eu2/eu2 or eu3-e1/eu3-e1 mutants is also affected by these plant mutations. The bacteria isolated from leaves of these soybean mutants have transient urease- and hydrogenase-deficient phenotypes that can be corrected by the addition of nickel to free-living cultures. The same bacterium growing on wild-type soybeans or on urease mutants eu1-sun/ eu1-sun or eu4/eu4, each deficient in only one urease isozyme, are urease-positive. These results suggest that the bacterium living on the eu2/eu2 or eu3-e1/eu3-e1 mutant is unable to produce an active urease or hydrogenase because it is effectively starved for nickel. We infer that mutations at Eu2 or Eu3 result in defects in nickel metabolism but not in Ni(2+) uptake or transport, because eu2/eu2 and eu3-e1/eu3-e1 mutants exhibit normal uptake of 63NiCl2. Moreover, wild-type plants grafted on mutant rootstocks produce seeds with fully active urease, indicating unimpeded transport of nickel through mutant roots and stems</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16668768</pmid><doi>10.1104/pp.98.3.942</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE BACTERIA Bacteriology Biological and medical sciences Callus FENOTIPOS FEUILLE Fundamental and applied biological sciences. Psychology Genetic mutation Genotypes GLYCINE MAX HOJAS Leaves Metabolism. Enzymes METABOLISME METABOLISMO Microbiology MUTANT MUTANTES NICKEL NIQUEL OXIDORREDUCTASAS OXYDOREDUCTASE PHENOTYPE Phenotypes Plants Scions Seedlings Soybeans UREASA UREASE |
title | Urease-null and hydrogenase-null phenotypes of a phylloplane bacterium reveal altered nickel metabolism in two soybean mutants |
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