Simultaneous determination of Fe, P, Ca, Mg, Zn, and Cu in whole blood by two-jet plasma atomic emission spectrometry

Availability of many biological samples in ample quantity for biomedical investigations sometimes is very restricted. Therefore, there is the need for the simple techniques allowing the analysis of small amount samples. In the present work the two-jet plasma atomic emission spectrometry techniques f...

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Bibliographic Details
Published in:Talanta (Oxford) 2010-03, Vol.80 (5), p.2187-2190
Main Authors: Zaksas, Natalia P., Gerasimov, Vladimir A., Nevinsky, Georgy A.
Format: Article
Language:English
Subjects:
Animals
Blood Chemical Analysis - methods
Calcium - blood
Cattle
Copper - blood
Direct analysis
Essential elements
Graphite powder
Humans
Iron - blood
Magnesium - blood
Metals - blood
Mice
Phosphorus - blood
Rats
Sample carbonization
Spectrophotometry, Atomic - methods
Two-jet plasma
Whole blood
Zinc - blood
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Summary:Availability of many biological samples in ample quantity for biomedical investigations sometimes is very restricted. Therefore, there is the need for the simple techniques allowing the analysis of small amount samples. In the present work the two-jet plasma atomic emission spectrometry techniques for the determination of Fe, P, Ca, Mg, Zn, and Cu in whole blood are proposed. The first technique is developed for direct analysis of freeze-dried blood. The sample preparation consisted in a dilution of blood powder (particle size 20 μm or less) with a spectroscopic buffer (graphite powder containing 15 wt.% NaCl). For the analysis of liquid whole blood, previous carbonization (not ashing) of blood evaporated on graphite powder was applied. Calibration samples based on graphite powder containing 15 wt.% NaCl were used. The validation of the techniques was confirmed by the use of different sample preparation procedures (wet acid digestion and dry carbonization), the analysis of IAEA A-13 reference material (freeze-dried bovine whole blood), and the comparison of the results obtained by the proposed technique with the results of the stripping voltammetry technique. Just 20–50 μL of whole blood is quite enough for all determinations. The proposed techniques were successfully applied for the simultaneous determination of Fe, P, Ca, Mg, Zn, and Cu in whole blood of living experimental rats and mice and human blood.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2009.10.046