Presence, characterization, and genotype profiles of Mycobacterium avium subspecies paratuberculosis from unpasteurized individual and pooled milk, commercial pasteurized milk, and milk products in India by culture, PCR, and PCR-REA methods

• Published in: International journal of infectious diseases 2010-02, Vol.14 (2), p.e121-e126
• Main Authors: Shankar, H, Singh, S.V, Singh, P.K, Singh, A.V, Sohal, J.S, Greenstein, R.J
• Format: Article
• Language: English
• Subjects: Animals; Bacterial Typing Techniques; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - epidemiology; Cattle Diseases - microbiology; Colony Count, Microbial; Culture; Culture Media; Dairy products; Dairy Products - microbiology; DNA Transposable Elements - genetics; DNA, Bacterial - analysis; DNA, Bacterial - genetics; Food Contamination; Genotype; Humans; India; Infectious Disease; IS 1311 PCR-REA; Johne's disease; Milk; Milk - microbiology; Mycobacterium avium subsp. paratuberculosis - classification; Mycobacterium avium subsp. paratuberculosis - genetics; Mycobacterium avium subsp. paratuberculosis - isolation & purification; Mycobacterium avium subspecies paratuberculosis; Paratuberculosis - diagnosis; Paratuberculosis - epidemiology; Paratuberculosis - microbiology; Pasteurized; Polymerase Chain Reaction - methods; Pulmonary/Respiratory; Restriction Mapping - methods
• ISSN: 1201-9712; 1878-3511
• DOI: 10.1016/j.ijid.2009.03.031

Summary Background Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne's disease in ruminants, a chronic enteritis evocative of human inflammatory bowel disease. In industrialized countries MAP has been cultured from pasteurized milk, compounding the increasing concern that MAP may be zoonotic. The purpose of this study was to evaluate commercially available unpasteurized and pasteurized milk and its products for the presence of viable MAP or MAP DNA from an area of northern India with a population of 150 million people. Methods We studied 43 samples (16 unpasteurized, 27 pasteurized) purchased in Mathura, Agra, or New Delhi, for the presence of MAP by culture or by PCR for IS 900 MAP DNA. Positives results were confirmed as MAP by restriction endonuclease analysis and/or DNA sequencing. Results Colonies appeared in 1.5–20 months post-inoculation. Of the unpasteurized samples, 44% (7/16) were MAP culture-positive and 6% (1/16) were positive for IS 900 MAP DNA. Of the pasteurized samples, 67% (18/27) were MAP culture-positive and 33% (9/27) were IS 900 -positive. Subsequently, 100% (25/25) of the cultured colonies were IS 900 and IS 1311 MAP DNA-positive. Conclusions This is the first report from a developing country of MAP cultured from both pasteurized and unpasteurized milk and milk products. Thus we corroborate the presence of viable MAP in the food chain reported from industrialized countries. With the increasing concern that MAP may be zoonotic, these findings have major implications for healthcare in India. The decreased sensitivity in detecting MAP DNA by PCR directly from milk should be ascribed to our employing only one set of PCR primers.