Retronectin enhances lentivirus-mediated gene delivery into hematopoietic progenitor cells

• Published in: Biologicals 2009-08, Vol.37 (4), p.203-209
• Main Authors: Lee, Hyun-Joo, Lee, Yong-Soo, Kim, Hye-Sun, Kim, Yu-Kyung, Kim, Jae-Hwan, Jeon, Seong-Ho, Lee, Hyeon-Woo, Kim, Sinae, Miyoshi, Hiroyuki, Chung, Hyung-Min, Kim, Dong-Ku
• Format: Article
• Language: English
• Subjects: Animals; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Cells, Cultured; Colony-forming activity; Drug Evaluation, Preclinical; Fibronectins - chemistry; Fibronectins - pharmacology; Gene delivery; Gene Transfer Techniques; Hematopoietic progenitor cells; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - metabolism; Humans; Intra-bone marrow injection; Lentivirus; Lentivirus - genetics; Lentivirus - physiology; Mice; Mice, Inbred C57BL; Multipotent Stem Cells - drug effects; Multipotent Stem Cells - metabolism; Peptide Fragments - pharmacology; Protein Structure, Tertiary; Recombinant Proteins - pharmacology; Retronectin; Up-Regulation
• ISSN: 1045-1056; 1095-8320
• DOI: 10.1016/j.biologicals.2009.01.008

Genetic modification of hematopoietic stem cells holds great promise in the treatment of hematopoietic disorders. However, clinical application of gene delivery has been limited, in part, by low gene transfer efficiency. To overcome this problem, we investigated the effect of retronectin (RN) on lentiviral-mediated gene delivery into hematopoietic progenitor cells (HPCs) derived from bone marrow both in vitro and in vivo. RN has been shown to enhance transduction by promoting colocalization of lentivirus and target cells. We found that RN enhanced lentiviral transfer of the VENUS transgene into cultured c-Kit + Lin − HPCs. As a complementary approach, in vivo gene delivery was performed by subjecting mice to intra-bone marrow injection of lentivirus or a mixture of RN and lentivirus. We found that co-injection with RN increased the number of VENUS-expressing c-Kit + Lin − HPCs in bone marrow by 2-fold. Further analysis of VENUS expression in colony-forming cells from the bone marrow of these animals revealed that RN increased gene delivery among these cells by 4-fold. In conclusion, RN is effective in enhancing lentivirus-mediated gene delivery into HPCs.