Amplification of Bovine Viral Diarrhoea Virus Introduced into an In Vitro Embryo Production System Via Oocytes from Persistently Infected Cattle

The purpose of this study was to determine whether or not embryos derived from in vitro fertilization of oocytes from persistently infected (PI) cattle would contain infectious virus. Three in vitro embryo production treatment groups were assessed: 1) oocytes and uterine tubal cells (UTC) free of bo...

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Bibliographic Details
Published in:Reproduction in domestic animals 2009-06, Vol.44 (3), p.532-535
Main Authors: Marley, MSD, Givens, MD, Galik, PK, Riddell, KP, Stringfellow, DA
Format: Article
Language:English
Subjects:
Animal diseases
Animal reproduction
Animals
Biological and medical sciences
Cattle
Cattle - embryology
Culture Media
Diarrhea Viruses, Bovine Viral - growth & development
Embryo, Mammalian - virology
Embryonic Development
Embryos
Epithelial Cells - physiology
Fallopian Tubes - cytology
Female
Fertilization in Vitro - veterinary
Follicular Fluid - virology
Fundamental and applied biological sciences. Psychology
Infectious diseases
Mammalian reproduction. General aspects
Oocytes - growth & development
Oocytes - virology
Reproductive technologies
Vertebrates: reproduction
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Summary:The purpose of this study was to determine whether or not embryos derived from in vitro fertilization of oocytes from persistently infected (PI) cattle would contain infectious virus. Three in vitro embryo production treatment groups were assessed: 1) oocytes and uterine tubal cells (UTC) free of bovine viral diarrhoea virus (BVDV) (negative control), 2) oocytes free of BVDV fertilized and cultured in media containing UTC obtained from PI heifers, and 3) oocytes from PI heifers fertilized and cultured in media containing UTC free of BVDV. The developmental media, UTC and embryos (individual or groups of five) were assayed for virus. Virus was not isolated from any samples in treatment group 1. As shown in previous studies, a proportion of embryo samples were positive for BVDV in treatment group 2. In treatment group 3, the virus associated with the oocytes contaminated the developmental media and infected susceptible co-culture cells used during fertilization and culture. In addition, 65% (11/17) of the degenerated ova from treatment group 3 had infectious virus associated with them. While none of the ova developed into transferable embryos, the study did confirm that use of oocytes from PI cows could lead to amplification of BVDV and cross contamination during in vitro embryo production.
ISSN:0936-6768
1439-0531
DOI:10.1111/j.1439-0531.2008.01108.x