Saccharomyces cerevisiae and Candida albicans Stimulate Cytokine Secretion from Human Neutrophil-Like HL-60 Cells Differentiated with Retinoic Acid or Dimethylsulfoxide

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-12, Vol.73 (12), p.2600-2608
• Main Authors: SAEGUSA, Shizue, TOTSUKA, Mamoru, KAMINOGAWA, Shuichi, HOSOI, Tomohiro
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Candida albicans; Candida albicans - immunology; Candida albicans - metabolism; Cell Differentiation - drug effects; cytokine; Cytokines - genetics; Cytokines - metabolism; Cytokines - secretion; Dimethyl Sulfoxide - pharmacology; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Gene Expression Regulation - immunology; HL-60 Cells; Hot Temperature; Humans; Lectins, C-Type; Membrane Proteins - genetics; Membrane Proteins - metabolism; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; neutrophil-like HL-60 cells; Neutrophils - cytology; Neutrophils - drug effects; Neutrophils - immunology; Neutrophils - secretion; RNA, Messenger - genetics; RNA, Messenger - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - immunology; Saccharomyces cerevisiae - metabolism; Toll-like receptor; Toll-Like Receptors - genetics; Toll-Like Receptors - metabolism; Tretinoin - pharmacology
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.90410

We investigated whether non-pathogenic Saccharomyces cerevisiae and human commensal opportunistic pathogenic Candida albicans stimulate cytokine responses of human neutrophil-like HL-60 cells pre-treated with either 1 μM retinoic acid or 1.25% dimethyl sulfoxide (DMSO). Intact and heat-killed S. cerevisiae enhanced secretion of interleukin (IL)-1β, IL-6, IL-8, IL-12, IL-18, MCP-1/CCL2 and TNF-α from retinoic acid-treated HL-60 cells, accompanied by alterations in mRNA expression of the cytokines. Heat-killed C. albicans promoted secretion of IL-6, IL-8, IL-12, MCP-1 and TNF-α, while intact C. albicans slightly enhanced secretion of IL-1β, IL-8 and IL-18. In response to yeast stimuli, retinoic acid-treated HL-60 cells generally secreted cytokines more strongly than DMSO-treated HL-60 cells. Gene expression levels of Toll-like receptor (TLR)1, TLR2, TLR4, TLR6 and dectin-1 in HL-60 cells were additionally affected by retinoic acid or DMSO and by co-culturing with S. cerevisiae or C. albicans. Our results suggest that both intact and heat-killed S. cerevisiae and C. albicans induce cytokine responses of neutrophils in the intestine, and stimulate host immune function.