Application of photon correlation spectroscopy as a technique for detecting culture contamination

The application of photon correlation spectroscopy (PCS) to detect culture contamination in chemostats was studied. It was found that the presence of a given particle size in a population of particles of a different size could be detected, but this ability was strongly dependent on particles of a di...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1991-10, Vol.38 (8), p.929-940
Main Authors: Jepras, R. I., Perkins, E. A., Rarity, J., Carr, R. J. G., Clarke, D. J., Atkinson, T.
Format: Article
Language:English
Subjects:
Biological and medical sciences
cell culture
culture contamination
dynamic light scattering
Fundamental and applied biological sciences. Psychology
light scattering
microorganisms
mixed culture
Molecular biophysics
particle sizing
photon correlation spectroscopy
spectroscopy
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Summary:The application of photon correlation spectroscopy (PCS) to detect culture contamination in chemostats was studied. It was found that the presence of a given particle size in a population of particles of a different size could be detected, but this ability was strongly dependent on particles of a different size could be detected, but this ability was strongly dependent on particle size difference and was most sensitive when contaminants are larger than the host. The inherent polydisparity of actively growing and dividing microbial cells negates any advantage in the use of multi‐angle PCS to detect contaminants.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.260380814