How to improve the success rate of mouse cloning technology

It has now been 13 years since the first cloned mammal Dolly the sheep was generated from somatic cells using nuclear transfer (SCNT). Since then, this technique has been considered an important tool not only for animal reproduction but also for regenerative medicine. However, the success rate is st...

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Bibliographic Details
Published in:Journal of Reproduction and Development 2010, Vol.56(1), pp.20-30
Main Authors: van Thuan, N., Konkuk Univ., Seoul (Korea R.), Kishigami, S, Wakayama, T
Format: Article
Language:English
Subjects:
ANIMAL
ANIMALES
ANIMALS
Cellular Reprogramming - physiology
CLONACION
CLONAGE
Clone
Clone Cells - physiology
CLONING
Cloning, Organism - methods
Embryonic Stem Cells - physiology
Epigenesis, Genetic
Extinction, Biological
Female
GENETIC DISORDERS
GENETIC ENGINEERING
GENIE GENETIQUE
http://www.fao.org/aos/agrovoc#c_15952
http://www.fao.org/aos/agrovoc#c_15974
http://www.fao.org/aos/agrovoc#c_24120
http://www.fao.org/aos/agrovoc#c_3217
http://www.fao.org/aos/agrovoc#c_444
http://www.fao.org/aos/agrovoc#c_4795
INGENIERIA GENETICA
MALFORMACION
MALFORMATION
MALFORMATIONS
MICE
ntES cell
Nuclear Transfer
Nuclear Transfer Techniques
Oocytes - physiology
Pluripotent Stem Cells - physiology
RATON
Reprogramming
SOURIS
TRASTORNOS GENETICOS
TROUBLE GENETIQUE
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Summary:It has now been 13 years since the first cloned mammal Dolly the sheep was generated from somatic cells using nuclear transfer (SCNT). Since then, this technique has been considered an important tool not only for animal reproduction but also for regenerative medicine. However, the success rate is still very low and the mechanisms involved in genomic reprogramming are not yet clear. Moreover, the NT technique requires donated fresh oocyte, which raises ethical problems for production of human cloned embryo. For this reason, the use of induced pluripotent stem cells for genomic reprogramming and for regenerative medicine is currently a hot topic in this field. However, we believe that the NT approach remains the only valid way for the study of reproduction and basic biology. For example, only the NT approach can reveal dynamic and global modifications in the epigenome without using genetic modification, and it can generate offspring from a single cell or even a frozen dead body. Thanks to much hard work by many groups, cloning success rates are increasing slightly year by year, and NT cloning is now becoming a more applicable method. This review describes how to improve the efficiency of cloning, the establishment of clone-derived embryonic stem cells and further applications.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.09-221A