A Microfluidic System to Evaluate Intestinal Absorption

Intestinal absorption rates vary with the nature of the substances involved. In-vitro experiments with cell culture inserts are often conducted to evaluate the intestinal absorption rate. These inserts, however, require large amounts of cells, samples, and culture media, and take a long time to eval...

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Bibliographic Details
Published in:Analytical Sciences 2009/12/10, Vol.25(12), pp.1403-1407
Main Authors: IMURA, Yuki, ASANO, Yasuyuki, SATO, Kiichi, YOSHIMURA, Etsuro
Format: Article
Language:English
Subjects:
Analytical Chemistry
Caco-2 Cells
Cell Culture Techniques
Chemistry
Humans
Intestinal Absorption
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Permeability
Time Factors
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Summary:Intestinal absorption rates vary with the nature of the substances involved. In-vitro experiments with cell culture inserts are often conducted to evaluate the intestinal absorption rate. These inserts, however, require large amounts of cells, samples, and culture media, and take a long time to evaluate. To overcome these problems, we developed a microchip-based system that mimics the intestine. The microchip was composed of a glass slide, a permeable membrane, and polydimethylsiloxane (PDMS) sheets, which contained microchannels made by photolithography; Caco-2 cells were cultured on the membrane in the microchip. The system was regulated with a microsyringe pump. We conducted permeation tests; cyclophosphamide, which can permeate the intestinal barrier, displayed a high permeability coefficient and Lucifer yellow, which cannot be absorbed at the intestinal wall, displayed a low permeability coefficient. These results were consistent with those obtained using a conventional method, which supports the validity of our new system. The system realized an 80% reduction of cell consumption.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.25.1403