Myoblasts Differentiated From Adipose-derived Stem Cells to Treat Stress Urinary Incontinence

Objectives To investigate the application of adipose-derived stem cell (ADSC) technology in the treatment of stress incontinence. Methods The vaginal balloon dilatation method was used to establish an animal model of stress incontinence (in 20 female Sprague–Dawley rats), which was further examined...

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Published in:Urology (Ridgewood, N.J.) N.J.), 2010-03, Vol.75 (3), p.718-723
Main Authors: Fu, Qiang, Song, Xiao-Fei, Liao, Guo-Long, Deng, Chen-Liang, Cui, Lei
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Animals
Biological and medical sciences
Cell Differentiation
Disease Models, Animal
Female
Medical sciences
Myoblasts - cytology
Nephrology. Urinary tract diseases
Rats
Rats, Sprague-Dawley
Stem Cell Transplantation
Stem Cells - cytology
Urinary Incontinence, Stress - surgery
Urinary system involvement in other diseases. Miscellaneous
Urinary tract. Prostate gland
Urology
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Summary:Objectives To investigate the application of adipose-derived stem cell (ADSC) technology in the treatment of stress incontinence. Methods The vaginal balloon dilatation method was used to establish an animal model of stress incontinence (in 20 female Sprague–Dawley rats), which was further examined by urodynamics and histology. Endogenous rat ADSCs were collected and induced into myoblasts with 5-Aza induction technology in vitro. The identity of myoblasts was confirmed through immunofluorescence labeling with desmin and myosin. Induced cells were injected into the posterior urethral muscularis in the bladder neck of animals with stress incontinence. The effects were examined after 1 and 3 months by urodynamics and histology. Untreated ADSCs were also implanted as a method of control. Results Both maximal bladder capacity and leak point pressure significantly increased after 1 and 3 months postimplantation, compared with the control ( P
ISSN:0090-4295
1527-9995
DOI:10.1016/j.urology.2009.10.003