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Development and characterization of polymorphic microsatellite markers in Linum usitatissimum
Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were found to be polymorphic, with the number of alleles per locus ranging from two to six, an...
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Published in: | Journal of plant research 2010-01, Vol.123 (1), p.119-123 |
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description | Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were found to be polymorphic, with the number of alleles per locus ranging from two to six, and observed and expected heterozygosities ranging from 0.125 to 0.375 (mean 0.013) and from 0.233 to 0.842 (mean 0.601), respectively. These polymorphic new microsatellite loci will be useful for genetic linkage map construction, germplasm classification and identification, gene identification and quantitative trait loci mapping, and marker-assisted selection in breeding in L. usitatissimum. |
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Inst. of Bast Fiber Crops ; Long, S ; He, D ; Li, X ; Wang, Y ; Liu, J ; Chen X</creator><creatorcontrib>Deng, X., Chinese Academy of Agricultural Sciences, Changsha (China). Inst. of Bast Fiber Crops ; Long, S ; He, D ; Li, X ; Wang, Y ; Liu, J ; Chen X</creatorcontrib><description>Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were found to be polymorphic, with the number of alleles per locus ranging from two to six, and observed and expected heterozygosities ranging from 0.125 to 0.375 (mean 0.013) and from 0.233 to 0.842 (mean 0.601), respectively. These polymorphic new microsatellite loci will be useful for genetic linkage map construction, germplasm classification and identification, gene identification and quantitative trait loci mapping, and marker-assisted selection in breeding in L. usitatissimum.</description><identifier>ISSN: 0918-9440</identifier><identifier>EISSN: 1618-0860</identifier><identifier>DOI: 10.1007/s10265-009-0271-3</identifier><identifier>PMID: 19882206</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Alleles ; Base Sequence ; Biomarkers ; Biomedical and Life Sciences ; Botany ; Cultivars ; Dinucleotide Repeats - genetics ; Flax - genetics ; GENE ; Gene Frequency - genetics ; Gene mapping ; GENES ; GENETIC MARKERS ; Genome, Plant - genetics ; Genomics ; http://www.fao.org/aos/agrovoc#c_24030 ; http://www.fao.org/aos/agrovoc#c_27583 ; http://www.fao.org/aos/agrovoc#c_3214 ; http://www.fao.org/aos/agrovoc#c_36574 ; http://www.fao.org/aos/agrovoc#c_4355 ; http://www.fao.org/aos/agrovoc#c_6088 ; http://www.fao.org/aos/agrovoc#c_8157 ; Life Sciences ; LINUM USITATISSIMUM ; MARCADORES GENETICOS ; MARQUEUR GENETIQUE ; MICROSATELITES ; MICROSATELLITE ; Microsatellite Repeats - genetics ; MICROSATELLITES ; Molecular Sequence Data ; NUCLEOTIDE SEQUENCE ; Plant Biochemistry ; Plant Ecology ; Plant Physiology ; Plant Sciences ; POLIMORFISMO ; POLYMORPHISM ; Polymorphism, Genetic - genetics ; POLYMORPHISME ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; Technical Note ; VARIEDADES ; VARIETE ; VARIETIES</subject><ispartof>Journal of plant research, 2010-01, Vol.123 (1), p.119-123</ispartof><rights>The Botanical Society of Japan and Springer 2009</rights><rights>The Botanical Society of Japan and Springer 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-970c263037c1180602aafaaecc92a31aeabea5a1418dce7563ecdde051ce0f7a3</citedby><cites>FETCH-LOGICAL-c418t-970c263037c1180602aafaaecc92a31aeabea5a1418dce7563ecdde051ce0f7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19882206$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deng, X., Chinese Academy of Agricultural Sciences, Changsha (China). Inst. of Bast Fiber Crops</creatorcontrib><creatorcontrib>Long, S</creatorcontrib><creatorcontrib>He, D</creatorcontrib><creatorcontrib>Li, X</creatorcontrib><creatorcontrib>Wang, Y</creatorcontrib><creatorcontrib>Liu, J</creatorcontrib><creatorcontrib>Chen X</creatorcontrib><title>Development and characterization of polymorphic microsatellite markers in Linum usitatissimum</title><title>Journal of plant research</title><addtitle>J Plant Res</addtitle><addtitle>J Plant Res</addtitle><description>Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were found to be polymorphic, with the number of alleles per locus ranging from two to six, and observed and expected heterozygosities ranging from 0.125 to 0.375 (mean 0.013) and from 0.233 to 0.842 (mean 0.601), respectively. These polymorphic new microsatellite loci will be useful for genetic linkage map construction, germplasm classification and identification, gene identification and quantitative trait loci mapping, and marker-assisted selection in breeding in L. usitatissimum.</description><subject>Alleles</subject><subject>Base Sequence</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Botany</subject><subject>Cultivars</subject><subject>Dinucleotide Repeats - genetics</subject><subject>Flax - genetics</subject><subject>GENE</subject><subject>Gene Frequency - genetics</subject><subject>Gene mapping</subject><subject>GENES</subject><subject>GENETIC MARKERS</subject><subject>Genome, Plant - genetics</subject><subject>Genomics</subject><subject>http://www.fao.org/aos/agrovoc#c_24030</subject><subject>http://www.fao.org/aos/agrovoc#c_27583</subject><subject>http://www.fao.org/aos/agrovoc#c_3214</subject><subject>http://www.fao.org/aos/agrovoc#c_36574</subject><subject>http://www.fao.org/aos/agrovoc#c_4355</subject><subject>http://www.fao.org/aos/agrovoc#c_6088</subject><subject>http://www.fao.org/aos/agrovoc#c_8157</subject><subject>Life Sciences</subject><subject>LINUM USITATISSIMUM</subject><subject>MARCADORES GENETICOS</subject><subject>MARQUEUR GENETIQUE</subject><subject>MICROSATELITES</subject><subject>MICROSATELLITE</subject><subject>Microsatellite Repeats - genetics</subject><subject>MICROSATELLITES</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>Plant Biochemistry</subject><subject>Plant Ecology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>POLIMORFISMO</subject><subject>POLYMORPHISM</subject><subject>Polymorphism, Genetic - genetics</subject><subject>POLYMORPHISME</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>Technical Note</subject><subject>VARIEDADES</subject><subject>VARIETE</subject><subject>VARIETIES</subject><issn>0918-9440</issn><issn>1618-0860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNp9kE1v1DAQhi0EokvhB3AAWVw4BWbsTewcUfnWSnCAI7KmzqR1SeJgJ0jl1-NVVqrEgZMt-XlfzzxCPEV4hQDmdUZQTV0BtBUog5W-J3bYoK3ANnBf7KAt93a_hzPxKOcbADR1ax-KM2ytVQqanfjxln_zEOeRp0XS1El_TYn8win8oSXEScZeznG4HWOar4OXY_ApZlp4GMLCcqT0k1OWYZKHMK2jXHNYSjDnMK7jY_GgpyHzk9N5Lr6_f_ft4mN1-PLh08WbQ-X3aJeqNeBVo0Ebj2ihAUXUE7H3rSKNxHTJVBMWuPNs6kaz7zqGGj1Db0ifi5db75zir5Xz4saQfRmRJo5rdkZrq3UNqpAv_iFv4pqmMpxTWFttEHWBcIOOq-bEvZtTKJveOgR3NO82866Yd0fz7ph5fipeL0fu7hIn1QVQG5DL03TF6e7n_7U-20I9RUdXKWT3-auCQgNYbPRfxLmZBA</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Deng, X., Chinese Academy of Agricultural Sciences, Changsha (China). Inst. of Bast Fiber Crops</creator><creator>Long, S</creator><creator>He, D</creator><creator>Li, X</creator><creator>Wang, Y</creator><creator>Liu, J</creator><creator>Chen X</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7ST</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20100101</creationdate><title>Development and characterization of polymorphic microsatellite markers in Linum usitatissimum</title><author>Deng, X., Chinese Academy of Agricultural Sciences, Changsha (China). Inst. of Bast Fiber Crops ; Long, S ; He, D ; Li, X ; Wang, Y ; Liu, J ; Chen X</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-970c263037c1180602aafaaecc92a31aeabea5a1418dce7563ecdde051ce0f7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Alleles</topic><topic>Base Sequence</topic><topic>Biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Botany</topic><topic>Cultivars</topic><topic>Dinucleotide Repeats - genetics</topic><topic>Flax - genetics</topic><topic>GENE</topic><topic>Gene Frequency - genetics</topic><topic>Gene mapping</topic><topic>GENES</topic><topic>GENETIC MARKERS</topic><topic>Genome, Plant - genetics</topic><topic>Genomics</topic><topic>http://www.fao.org/aos/agrovoc#c_24030</topic><topic>http://www.fao.org/aos/agrovoc#c_27583</topic><topic>http://www.fao.org/aos/agrovoc#c_3214</topic><topic>http://www.fao.org/aos/agrovoc#c_36574</topic><topic>http://www.fao.org/aos/agrovoc#c_4355</topic><topic>http://www.fao.org/aos/agrovoc#c_6088</topic><topic>http://www.fao.org/aos/agrovoc#c_8157</topic><topic>Life Sciences</topic><topic>LINUM USITATISSIMUM</topic><topic>MARCADORES GENETICOS</topic><topic>MARQUEUR GENETIQUE</topic><topic>MICROSATELITES</topic><topic>MICROSATELLITE</topic><topic>Microsatellite Repeats - genetics</topic><topic>MICROSATELLITES</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>Plant Biochemistry</topic><topic>Plant Ecology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>POLIMORFISMO</topic><topic>POLYMORPHISM</topic><topic>Polymorphism, Genetic - genetics</topic><topic>POLYMORPHISME</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>Technical Note</topic><topic>VARIEDADES</topic><topic>VARIETE</topic><topic>VARIETIES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deng, X., Chinese Academy of Agricultural Sciences, Changsha (China). Inst. of Bast Fiber Crops</creatorcontrib><creatorcontrib>Long, S</creatorcontrib><creatorcontrib>He, D</creatorcontrib><creatorcontrib>Li, X</creatorcontrib><creatorcontrib>Wang, Y</creatorcontrib><creatorcontrib>Liu, J</creatorcontrib><creatorcontrib>Chen X</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of plant research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, X., Chinese Academy of Agricultural Sciences, Changsha (China). Inst. of Bast Fiber Crops</au><au>Long, S</au><au>He, D</au><au>Li, X</au><au>Wang, Y</au><au>Liu, J</au><au>Chen X</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and characterization of polymorphic microsatellite markers in Linum usitatissimum</atitle><jtitle>Journal of plant research</jtitle><stitle>J Plant Res</stitle><addtitle>J Plant Res</addtitle><date>2010-01-01</date><risdate>2010</risdate><volume>123</volume><issue>1</issue><spage>119</spage><epage>123</epage><pages>119-123</pages><issn>0918-9440</issn><eissn>1618-0860</eissn><abstract>Thirty-five microsatellite loci were isolated and characterized in Linum usitatissimum using enriched genomic libraries. These loci were screened in eight cultivars from different countries and regions and were found to be polymorphic, with the number of alleles per locus ranging from two to six, and observed and expected heterozygosities ranging from 0.125 to 0.375 (mean 0.013) and from 0.233 to 0.842 (mean 0.601), respectively. These polymorphic new microsatellite loci will be useful for genetic linkage map construction, germplasm classification and identification, gene identification and quantitative trait loci mapping, and marker-assisted selection in breeding in L. usitatissimum.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>19882206</pmid><doi>10.1007/s10265-009-0271-3</doi><tpages>5</tpages></addata></record> |
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subjects | Alleles Base Sequence Biomarkers Biomedical and Life Sciences Botany Cultivars Dinucleotide Repeats - genetics Flax - genetics GENE Gene Frequency - genetics Gene mapping GENES GENETIC MARKERS Genome, Plant - genetics Genomics http://www.fao.org/aos/agrovoc#c_24030 http://www.fao.org/aos/agrovoc#c_27583 http://www.fao.org/aos/agrovoc#c_3214 http://www.fao.org/aos/agrovoc#c_36574 http://www.fao.org/aos/agrovoc#c_4355 http://www.fao.org/aos/agrovoc#c_6088 http://www.fao.org/aos/agrovoc#c_8157 Life Sciences LINUM USITATISSIMUM MARCADORES GENETICOS MARQUEUR GENETIQUE MICROSATELITES MICROSATELLITE Microsatellite Repeats - genetics MICROSATELLITES Molecular Sequence Data NUCLEOTIDE SEQUENCE Plant Biochemistry Plant Ecology Plant Physiology Plant Sciences POLIMORFISMO POLYMORPHISM Polymorphism, Genetic - genetics POLYMORPHISME SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE Technical Note VARIEDADES VARIETE VARIETIES |
title | Development and characterization of polymorphic microsatellite markers in Linum usitatissimum |
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