Comparison of Early Ileal Invasion by Salmonella enterica Serovars Choleraesuis and Typhimurium

• Published in: Veterinary pathology 2003-07, Vol.40 (4), p.371-375
• Main Authors: Meyerholz, D. K., Stabel, T. J.
• Format: Article
• Language: English
• Subjects: Animals; Ileum - microbiology; Ileum - pathology; Ileum - ultrastructure; Intestinal Diseases - microbiology; Intestinal Diseases - pathology; Intestinal Diseases - veterinary; Intestinal Mucosa - microbiology; Intestinal Mucosa - pathology; Intestinal Mucosa - ultrastructure; Salmonella enterica - physiology; Salmonella enterica - ultrastructure; Salmonella Infections, Animal - microbiology; Salmonella Infections, Animal - pathology; Swine Diseases - microbiology; Swine Diseases - pathology
• ISSN: 0300-9858; 1544-2217
• DOI: 10.1354/vp.40-4-371

The mechanisms of Salmonella serovar-host specificity are not well defined. Pig ileal loops were used to compare phenotypic differences in early cellular invasion between non-host-adapted Salmonella serovar Typhimurium (SsT) and host-adapted Salmonella serovar Choleraesuis (SsC). By 10 minutes postinoculation, both serovars invaded a small number of M cells, enterocytes, and goblet cells. Multiple SsC organisms (up to 6 per cell) simultaneously invaded M cells, whereas SsT often invaded as one to two organisms per M cell. Internalization of both serovars resulted in vacuoles containing a single bacterium. The follicle-associated epithelium (FAE) of SsC-inoculated loops responded with more filopodia and lamellipodia although exhibiting less cell swelling than SsT. Additionally, SsT showed an enhanced affinity for sites of cell extrusion compared with SsC at 60 minutes. These results suggest: 1) both SsC and SsT exhibit non-cell-specific invasion as early as 10 minutes postinoculation, 2) Salmonella serovars exhibit differences in early invasion of FAE and M cells, and 3) cells undergoing extrusion may provide a site for preferential adherence by SsT and SsC.