Resonant Mie scattering in infrared spectroscopy of biological materials - understanding the 'dispersion artefact'

Infrared spectroscopic cytology is potentially a powerful clinical tool. However, in order for it to be successful, practitioners must be able to extract reliably a pure absorption spectrum from a measured spectrum that often contains many confounding factors. The most intractable problem to date is...

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Bibliographic Details
Published in:Analyst (London) 2009-08, Vol.134 (8), p.1586-1593
Main Authors: BASSAN, Paul, BYRNE, Hugh J, BONNIER, Franck, LEE, Joe, DUMAS, Paul, GARDNER, Peter
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Exact sciences and technology
Manufactured Materials
Scattering, Radiation
Spectrometric and optical methods
Spectrophotometry, Infrared - methods
Spectroscopy, Fourier Transform Infrared - methods
Spectroscopy, Near-Infrared - methods
Spectrum Analysis
Surface Plasmon Resonance - methods
X-Ray Diffraction
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Summary:Infrared spectroscopic cytology is potentially a powerful clinical tool. However, in order for it to be successful, practitioners must be able to extract reliably a pure absorption spectrum from a measured spectrum that often contains many confounding factors. The most intractable problem to date is the, so called, dispersion artefact which most prominently manifests itself as a sharp decrease in absorbance on the high wavenumber side of the amide I band in the measured spectrum, exhibiting a derivative-like line shape. In this paper we use synchrotron radiation FTIR micro-spectroscopy to record spectra of mono-dispersed poly(methyl methacrylate) (PMMA) spheres of systematically varying size and demonstrate that the spectral distortions in the data can be understood in terms of resonant Mie scattering. A full understanding of this effect will enable us to develop strategies for deconvolving the scattering contribution and recovering the pure absorption spectrum, thus removing one of the last technological barriers to the development of clinical spectroscopic cytology.
ISSN:0003-2654
1364-5528
DOI:10.1039/b904808a