Heat-shock protein 70 favours human liver recovery from ischaemia-reperfusion

• Published in: European journal of clinical investigation 2003-06, Vol.33 (6), p.500-504
• Main Authors: Boeri, D., Dondero, F., Storace, D., Maiello, M., Pasqualini, M., Pellicci, R.
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Biological and medical sciences; Female; Fibrinogen - metabolism; Heat-shock protein 70; HSP70 Heat-Shock Proteins - metabolism; Humans; ischaemia-reperfusion; liver; Liver - blood supply; Liver - surgery; Liver, biliary tract, pancreas, portal circulation, spleen; Male; Medical sciences; Middle Aged; Myocardial Reperfusion Injury - blood; Myocardial Reperfusion Injury - metabolism; Recovery of Function; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the digestive system; Transaminases - adverse effects
• ISSN: 0014-2972; 1365-2362
• DOI: 10.1046/j.1365-2362.2003.01157.x

Background Pringle's manoeuvre controls excessive bleeding, but results in ischaemia‐reperfusion injury during liver surgery. Activation of the heat‐shock protein system of cell defense has been demonstrated after ischaemia‐reperfusion injury in animal tissues. The aim of the present study was to determine whether the ischaemia‐reperfusion accompanying hepatic surgery induces heat‐shock protein 70 (HSP70) in human liver and whether the induction of HSP70 is related to the recovery of liver function. Methods Heat‐shock protein 70 and γ‐actin mRNAs were assayed in the liver biopsies of 10 subjects undergoing partial hepatectomy for localized lesions. Measurements were performed before the Pringle's manoeuvre and at the end of the surgery. Transaminases and fibrinogen were measured before and at 12, 24 and 36 h following hepatectomy. Results After an average 40 ± 8‐min period of warm ischaemia, a significant increase of HSP70 mRNA (187 ± 67%, 2P