Twelve-week RBC storage

BACKGROUND: Better storage can improve RBC availability and safety. Optimizing RBC ATP production and minimizing hemolysis has allowed progressively longer storage. STUDY DESIGN AND METHODS: In the first study, 24 units of packed CPD RBCs were pooled in groups of four, realiquoted, and added to 300 ...

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Published in:Transfusion (Philadelphia, Pa.) Pa.), 2003-07, Vol.43 (7), p.867-872
Main Authors: Hess, John R., Hill, Heather R., Oliver, Cindy K., Lippert, Lloyd E., Rugg, Neeta, Joines, Amy D., Gormas, Jennifer F., Pratt, P. Gayle, Silverstein, Edward B., Greenwalt, Tibor J.
Format: Article
Language:English
Subjects:
2,3-Diphosphoglycerate - blood
Adenosine Triphosphate - blood
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Biological and medical sciences
Blood Donors
Blood Preservation - methods
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
Erythrocyte Transfusion
Erythrocytes
Female
Hemolysis
Humans
Male
Medical sciences
Sodium Chloride - analysis
Solutions
Time Factors
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Summary:BACKGROUND: Better storage can improve RBC availability and safety. Optimizing RBC ATP production and minimizing hemolysis has allowed progressively longer storage. STUDY DESIGN AND METHODS: In the first study, 24 units of packed CPD RBCs were pooled in groups of four, realiquoted, and added to 300 mL of one of four variants of experimental additive solution 76 (EAS‐76) containing 45, 40, 35, or 30 mEq per L NaCl. Units were sampled weekly for 12 weeks for morphologic and biochemical measures. In the second study, 10 volunteers donated 2 units of RBCs for a crossover comparison of Tc/Cr 24‐hour in vivo recovery of 6‐week storage in AS‐1 versus 12‐week storage in EAS‐76 variant 6 (EAS‐76v6) having 30 mEq per L NaCl. RESULTS: RBCs stored in the lower salt variants of EAS‐76 had higher concentrations of RBC ATP with less hemolysis and microvesiculation. RBC 2,3 DPG was preserved for two weeks. RBCs stored for 12 weeks in EAS‐76v6 exhibited 78 ± 4 percent 24‐hour in vivo recovery. CONCLUSIONS: It is possible to store RBCs for 12 weeks with acceptable recovery and 0.6 percent hemolysis and with normal 2,3 DPG concentrations for 2 weeks.
ISSN:0041-1132
1537-2995
DOI:10.1046/j.1537-2995.2003.00442.x