Matrine determination and pharmacokinetics in human plasma using LC/MS/MS

A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for the determination of matrine in human plasma extracted by isopropanol:ethyl acetate (v/v, 5:95). Rapid chromatographic separation was achieved in the mobile phase composition of 5-mM aqueous ammonium acetate and ace...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-10, Vol.877 (27), p.3253-3256
Main Authors: Zhang, Xiao-Lin, Xu, Hong-Rong, Chen, Wei-Li, Chu, Nan-Nan, Li, Xue-Ning, Liu, Gang-Yi, Yu, Chen
Format: Article
Language:English
Subjects:
Alkaloids - blood
Alkaloids - pharmacokinetics
Analysis
Analytical, structural and metabolic biochemistry
Area Under Curve
Biological and medical sciences
Calibration
Chromatography, Liquid - methods
Drug Stability
Fundamental and applied biological sciences. Psychology
General pharmacology
Humans
LC/MS/MS
Least-Squares Analysis
Matrine
Medical sciences
Pharmacokinetics
Pharmacology. Drug treatments
Quinolizines - blood
Quinolizines - pharmacokinetics
Reproducibility of Results
Sensitivity and Specificity
Sesquiterpenes - analysis
Tandem Mass Spectrometry - methods
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Summary:A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for the determination of matrine in human plasma extracted by isopropanol:ethyl acetate (v/v, 5:95). Rapid chromatographic separation was achieved in the mobile phase composition of 5-mM aqueous ammonium acetate and acetonitrile (v/v, 70:30) with a flow rate of 0.20 ml/min. Detection was carried out using positive-ion electrospray tandem mass spectrometry on a Sciex API3000. The method was accurate, specific and sensitive for the analysis of matrine in human plasma in the concentration range of 5–2000 ng/ml, when huperzine A was used as internal standard. The method facilitated a clinical pharmacokinetic study after oral administration of a single dose of matrine soft gelatin capsules (100, 200 and 400 mg) in a three-period crossover design. Dose-related linear trends were observed for the AUC 0− t and the C max of matrine. The t 1/2 and the T max of matrine were independent of the administered doses.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2009.08.026