Plasma polymerized non-fouling thin films for DNA immobilization

Development of DNA sensors has been an issue of great interest, owing to their potential applications in different fields, such as the diagnosis of infectious diseases, food quality control, or for environmental monitoring. Most DNA sensors involved the immobilization of single-stranded oligonucleot...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2009-10, Vol.25 (2), p.519-522
Main Authors: Chu, Li-Qiang, Knoll, Wolfgang, Förch, Renate
Format: Article
Language:English
Subjects:
Adsorption
Biological and medical sciences
Biosensing Techniques - instrumentation
Biotechnology
DNA - chemistry
DNA Probes - analysis
DNA Probes - chemistry
DNA sensor
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
General aspects
Hot Temperature
Immobilization techniques
Membranes, Artificial
Methods. Procedures. Technologies
Multifunctional surface
Non-fouling
Oligonucleotide Array Sequence Analysis - instrumentation
Plasma polymerization
Polymers - chemistry
Spectrometry, Fluorescence - instrumentation
Surface plasmon enhanced fluorescence spectroscopy (SPFS)
Surface Plasmon Resonance - instrumentation
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Summary:Development of DNA sensors has been an issue of great interest, owing to their potential applications in different fields, such as the diagnosis of infectious diseases, food quality control, or for environmental monitoring. Most DNA sensors involved the immobilization of single-stranded oligonucleotides, so-called DNA probes, onto the sensor surfaces. Here we report on a new approach for DNA probe immobilization using the streptavidin–biotin assembly coupled with a non-fouling thin film. Pulsed plasma polymerization of di(ethylene glycol) monovinyl ether (ppEO2) will result in non-fouling thin films, which are employed to immobilize streptavidin. By careful control of the thickness and the chemistry of ppEO2 films, the embedded streptavidin are able to bind the biotinylated oligonucleotides. The resulting DNA sensors show good resistance towards adsorption of both BSA and fibrinogen, and are employed to discriminate different DNA sequences from protein-containing sample solutions, as seen by surface plasmon enhanced fluorescence spectroscopy (SPFS). This result suggests that the present sensor is very promising for the detection of a DNA sequence from a complex solution.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2009.07.033