Slowing DNA Translocation through Nanopores Using a Solution Containing Organic Salts

One of the key challenges to nanopore DNA sequencing is to slow down DNA translocation. Here, we report that the translocation velocities of various DNA homo- and copolymers through protein pores could be significantly decreased by using electrolyte solutions containing organic salts. Using a butylm...

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Bibliographic Details
Published in:The journal of physical chemistry. B 2009-10, Vol.113 (40), p.13332-13336
Main Authors: de Zoysa, Ranulu Samanthi S, Jayawardhana, Dilani A, Zhao, Qitao, Wang, Deqiang, Armstrong, Daniel W, Guan, Xiyun
Format: Article
Language:English
Subjects:
B: Biophysical Chemistry
Biological Transport - physiology
Chlorides - chemistry
DNA - chemistry
DNA - metabolism
Imidazolines - chemistry
Lipid Bilayers - chemistry
Nanostructures
Organic Chemicals - chemistry
Salts - chemistry
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Summary:One of the key challenges to nanopore DNA sequencing is to slow down DNA translocation. Here, we report that the translocation velocities of various DNA homo- and copolymers through protein pores could be significantly decreased by using electrolyte solutions containing organic salts. Using a butylmethylimidazolium chloride (BMIM-Cl) solution instead of the commonly used KCl solution, DNA translocation rates on the order of hundreds of microseconds per nucleotide base were achieved. The much enhanced resolution of the nanopore coupled with different event blockage amplitudes produced by different nucleotides permits the convenient differentiation between various DNA molecules.
ISSN:1520-6106
1520-5207
DOI:10.1021/jp9040293