A novel method for processing resin-embedded specimens with metal implants for immunohistochemical labelling

A major technical problem in the processing of resin-embedded tissues is the adhesion of the tissue sample on glass slides for immunohistochemical labelling. We therefore established a novel protocol for processing such specimens with improved attachment of the tissue sample during resin removal (de...

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Bibliographic Details
Published in:Acta histochemica 2009-11, Vol.111 (6), p.538-542
Main Authors: Quentin, Thomas, Poppe, Andrea, Bär, Karin, Sigler, Albrecht, Foth, Rudi, Michel-Behnke, Ina, Paul, Thomas, Sigler, Matthias
Format: Article
Language:English
Subjects:
Glass - chemistry
Humans
Immunohistochemistry
Immunohistochemistry - methods
Methylmethacrylate embedding
Paraffin - chemistry
Silicone Elastomers - chemistry
Smooth muscle
Stented vessel
Stents
Surface Properties
Umbilical Cord - cytology
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Summary:A major technical problem in the processing of resin-embedded tissues is the adhesion of the tissue sample on glass slides for immunohistochemical labelling. We therefore established a novel protocol for processing such specimens with improved attachment of the tissue sample during resin removal (deplastification). In order to demonstrate the feasibility of the procedure we employed a panel of smooth muscle cell maturation markers. The technique makes use of a silicone glue (Elastosil E41; Wacker Chemie, München, Germany) to attach the tissue samples to the glass slides. This allows resin dissolution in xylene/2-methoxyethylacetate without detachment of the sample from the slide. Our results demonstrate successful immunohistochemical labelling with primary antibodies directed against: smooth muscle actin, smooth muscle myosin, h-caldesmon, desmin, vimentin and von Willebrand factor. In conclusion, we have established a new and successful method for resin-embedded sample adhesion on glass slides. The developed protocol is feasible for investigation of cells which are involved in intimal proliferation following stent implantation.
ISSN:0065-1281
1618-0372
DOI:10.1016/j.acthis.2008.04.001