Treatment with the 5-HT3 antagonist tropisetron modulates glucose-induced obesity in mice

• Published in: International Journal of Obesity 2009-12, Vol.33 (12), p.1339-1347
• Main Authors: Weber, S, Volynets, V, Kanuri, G, Bergheim, I, Bischoff, S C
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Body weight; Body Weight - drug effects; Body Weight - physiology; Calories; Carbohydrates; Care and treatment; Chronic illnesses; Dehydrogenase; Diabetes; Drinking water; Epidemiology; Fatty acids; Food; Gastrointestinal Motility - drug effects; Gastrointestinal Motility - physiology; Genetic aspects; Glucose; Glucose - metabolism; Glucose Transport Proteins, Facilitative - metabolism; Health Promotion and Disease Prevention; Hypotheses; Indoles - administration & dosage; Internal Medicine; Ketones; Laboratory animals; Medical sciences; Medicine; Medicine & Public Health; Metabolic Diseases; Methods; Mice; Mice, Inbred C57BL; Obesity; Obesity - metabolism; Obesity - prevention & control; original-article; Overweight; Physiological aspects; Prevalence studies (Epidemiology); Prevention; Proteins; Public Health; RNA, Messenger - metabolism; Serotonin; Serotonin Antagonists - administration & dosage; Sodium; Soft drinks; Sugar; Weight Gain - drug effects; Weight Gain - physiology
• ISSN: 0307-0565; 1476-5497
• DOI: 10.1038/ijo.2009.191

Objective: Sugar consumption has increased markedly over the last few decades and parallels the dramatic increase in overweight and obesity. Data obtained from animal studies suggest that the intestinal serotonergic system and herein particularly the serotonin receptor 3 (5-HT3R) may be involved in sugar detection and short-term control of food intake. Using a mouse model, we tested the hypothesis that blocking 5-HT3R prevents the development of sugar-induced obesity. Design: For 8 weeks, C57BL/J6 mice were offered either water containing 30% glucose or plain water in addition to normal chow. The effect of oral treatment with the 5-HT3R antagonist, tropisetron (0.2 mg kg −1 body weight), on body weight and caloric intake was studied. Results: Total caloric intake and weight gain were significantly increased in mice fed glucose compared with the control group. Tropisetron treatment reduced intestinal motility and almost completely blocked weight gain associated with glucose feeding; however, total caloric intake was not affected. The effect of tropisetron was not associated with a decreased expression of the intestinal and hepatic glucose transporters, SGLT1 (sodium-dependent glucose cotransporter) and Glut2 (glucose transporter 2); instead, the expression of these transporters was slightly increased by the 5-HT3R antagonist. However, expressions of carbohydrate responsive element binding protein and fatty acid synthase, as well as triglyceride levels in the liver were only enhanced in mice fed glucose, but remained unchanged at the level of the control group when mice were treated concomitantly with tropisetron. At the same time, β-hydroxybutyrate dehydrogenase mRNA expression and plasma levels of ketone bodies were significantly increased. Conclusion: Our results suggest that 5-HT3R is a new target for the modulation of hepatic glucose metabolism and for the prevention of obesity.