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Molecular Typing of Bacillus thuringiensis Serovars by RAPD-PCR
One hundred and twenty-six strains of Bacillus thuringiensis representing 57 serovars were allocated to 58 genomic types using random amplified polymorphic DNA (RAPD)-PCR patterns. Serovars darmstadiensis, israelensis, kenyae, kumamotoensis, kurstaki, morrisoni, pakistani, sotto, thuringiensis and t...
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| Published in: | Systematic and applied microbiology 2003-06, Vol.26 (2), p.254-261 |
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| Language: | English |
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| container_title | Systematic and applied microbiology |
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| creator | Gaviria Rivera, Adelaida M. Priest, Fergus G. |
| description | One hundred and twenty-six strains of
Bacillus thuringiensis representing 57 serovars were allocated to 58 genomic types using random amplified polymorphic DNA (RAPD)-PCR patterns. Serovars
darmstadiensis,
israelensis, kenyae,
kumamotoensis,
kurstaki,
morrisoni,
pakistani,
sotto,
thuringiensis and
tolworthi each encompassed identical or closely related strains. Despite this genomic homogeneity, most of these serovars also included at least one variant strain. Serovars
aizawai,
canadensis, entomocidus and
sotto biotype
dendrolimus, on the other hand, were genomically heterogeneous. Of the 57 serovars examined, 31 contained at least one strain with a closely related or identical RAPD pattern to a strain from a different serovar. We conclude that while the species is genomically diverse, the homogeneous serovars represent clonal lineages of successful insect pathogens. |
| doi_str_mv | 10.1078/072320203322346100 |
| format | article |
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Bacillus thuringiensis representing 57 serovars were allocated to 58 genomic types using random amplified polymorphic DNA (RAPD)-PCR patterns. Serovars
darmstadiensis,
israelensis, kenyae,
kumamotoensis,
kurstaki,
morrisoni,
pakistani,
sotto,
thuringiensis and
tolworthi each encompassed identical or closely related strains. Despite this genomic homogeneity, most of these serovars also included at least one variant strain. Serovars
aizawai,
canadensis, entomocidus and
sotto biotype
dendrolimus, on the other hand, were genomically heterogeneous. Of the 57 serovars examined, 31 contained at least one strain with a closely related or identical RAPD pattern to a strain from a different serovar. We conclude that while the species is genomically diverse, the homogeneous serovars represent clonal lineages of successful insect pathogens.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1078/072320203322346100</identifier><identifier>PMID: 12866852</identifier><identifier>CODEN: SAMIDF</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>Bacillus thuringiensis ; Bacillus thuringiensis - classification ; Bacillus thuringiensis - genetics ; Bacterial Typing Techniques - methods ; Biological and medical sciences ; clone ; DNA, Bacterial - genetics ; Epidemiology ; Fundamental and applied biological sciences. Psychology ; Genome, Bacterial ; Microbiology ; Mycology ; Pathogenicity, host-agent relations, miscellaneous strains, epidemiology ; Phylogeny ; Polymerase Chain Reaction ; Random Amplified Polymorphic DNA Technique ; RAPD ; Serotyping ; serovar ; Species Specificity ; Virology</subject><ispartof>Systematic and applied microbiology, 2003-06, Vol.26 (2), p.254-261</ispartof><rights>2003 Urban & Fischer Verlag</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Urban & Fischer Verlag Jun 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-36223923945b040cca47548a8dfa4eba247030cf8b703901d869e68fd64b86a13</citedby><cites>FETCH-LOGICAL-c506t-36223923945b040cca47548a8dfa4eba247030cf8b703901d869e68fd64b86a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14888055$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12866852$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gaviria Rivera, Adelaida M.</creatorcontrib><creatorcontrib>Priest, Fergus G.</creatorcontrib><title>Molecular Typing of Bacillus thuringiensis Serovars by RAPD-PCR</title><title>Systematic and applied microbiology</title><addtitle>Syst Appl Microbiol</addtitle><description>One hundred and twenty-six strains of
Bacillus thuringiensis representing 57 serovars were allocated to 58 genomic types using random amplified polymorphic DNA (RAPD)-PCR patterns. Serovars
darmstadiensis,
israelensis, kenyae,
kumamotoensis,
kurstaki,
morrisoni,
pakistani,
sotto,
thuringiensis and
tolworthi each encompassed identical or closely related strains. Despite this genomic homogeneity, most of these serovars also included at least one variant strain. Serovars
aizawai,
canadensis, entomocidus and
sotto biotype
dendrolimus, on the other hand, were genomically heterogeneous. Of the 57 serovars examined, 31 contained at least one strain with a closely related or identical RAPD pattern to a strain from a different serovar. We conclude that while the species is genomically diverse, the homogeneous serovars represent clonal lineages of successful insect pathogens.</description><subject>Bacillus thuringiensis</subject><subject>Bacillus thuringiensis - classification</subject><subject>Bacillus thuringiensis - genetics</subject><subject>Bacterial Typing Techniques - methods</subject><subject>Biological and medical sciences</subject><subject>clone</subject><subject>DNA, Bacterial - genetics</subject><subject>Epidemiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genome, Bacterial</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Pathogenicity, host-agent relations, miscellaneous strains, epidemiology</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>RAPD</subject><subject>Serotyping</subject><subject>serovar</subject><subject>Species Specificity</subject><subject>Virology</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqF0VlLxDAQAOAgiq7HH_BBiqBv1cnRdAqCrOsJiuLxHNI01Ui3XZOtsP_eLLsgKCgEBsI3wxyE7FI4opDjMeSMM2DAOWNcSAqwQgZUUkyhQLFKBnOQzsUG2QzhHYCKQtJ1skEZSokZG5DTu66xpm-0T55nE9e-Jl2dnGnjmqYPyfSt9_HP2Ta4kDxZ331qH5JyljwOH87Th9HjNlmrdRPszjJukZfLi-fRdXp7f3UzGt6mJgM5TbmMLRbxiawEAcZokWcCNVa1FrbUTOTAwdRYxlgArVAWVmJdSVGi1JRvkcNF3YnvPnobpmrsgrFNo1vb9UHlXDCKmP8LIyo45POK-z_ge9f7Ng6hGKDIMO4oIrZAxncheFuriXdj7WeKgpofQf0-QkzaW1buy7GtvlOWW4_gYAl0MLqpvW6NC99OICJkWXQnC2fjZj-d9SqYeAtjK-etmaqqc3_18QUadZ2B</recordid><startdate>20030601</startdate><enddate>20030601</enddate><creator>Gaviria Rivera, Adelaida M.</creator><creator>Priest, Fergus G.</creator><general>Elsevier GmbH</general><general>Elsevier</general><general>Elsevier Science Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030601</creationdate><title>Molecular Typing of Bacillus thuringiensis Serovars by RAPD-PCR</title><author>Gaviria Rivera, Adelaida M. ; Priest, Fergus G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-36223923945b040cca47548a8dfa4eba247030cf8b703901d869e68fd64b86a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Bacillus thuringiensis</topic><topic>Bacillus thuringiensis - classification</topic><topic>Bacillus thuringiensis - genetics</topic><topic>Bacterial Typing Techniques - methods</topic><topic>Biological and medical sciences</topic><topic>clone</topic><topic>DNA, Bacterial - genetics</topic><topic>Epidemiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genome, Bacterial</topic><topic>Microbiology</topic><topic>Mycology</topic><topic>Pathogenicity, host-agent relations, miscellaneous strains, epidemiology</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>RAPD</topic><topic>Serotyping</topic><topic>serovar</topic><topic>Species Specificity</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gaviria Rivera, Adelaida M.</creatorcontrib><creatorcontrib>Priest, Fergus G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gaviria Rivera, Adelaida M.</au><au>Priest, Fergus G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Typing of Bacillus thuringiensis Serovars by RAPD-PCR</atitle><jtitle>Systematic and applied microbiology</jtitle><addtitle>Syst Appl Microbiol</addtitle><date>2003-06-01</date><risdate>2003</risdate><volume>26</volume><issue>2</issue><spage>254</spage><epage>261</epage><pages>254-261</pages><issn>0723-2020</issn><eissn>1618-0984</eissn><coden>SAMIDF</coden><abstract>One hundred and twenty-six strains of
Bacillus thuringiensis representing 57 serovars were allocated to 58 genomic types using random amplified polymorphic DNA (RAPD)-PCR patterns. Serovars
darmstadiensis,
israelensis, kenyae,
kumamotoensis,
kurstaki,
morrisoni,
pakistani,
sotto,
thuringiensis and
tolworthi each encompassed identical or closely related strains. Despite this genomic homogeneity, most of these serovars also included at least one variant strain. Serovars
aizawai,
canadensis, entomocidus and
sotto biotype
dendrolimus, on the other hand, were genomically heterogeneous. Of the 57 serovars examined, 31 contained at least one strain with a closely related or identical RAPD pattern to a strain from a different serovar. We conclude that while the species is genomically diverse, the homogeneous serovars represent clonal lineages of successful insect pathogens.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><pmid>12866852</pmid><doi>10.1078/072320203322346100</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0723-2020 |
| ispartof | Systematic and applied microbiology, 2003-06, Vol.26 (2), p.254-261 |
| issn | 0723-2020 1618-0984 |
| language | eng |
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| source | Elsevier |
| subjects | Bacillus thuringiensis Bacillus thuringiensis - classification Bacillus thuringiensis - genetics Bacterial Typing Techniques - methods Biological and medical sciences clone DNA, Bacterial - genetics Epidemiology Fundamental and applied biological sciences. Psychology Genome, Bacterial Microbiology Mycology Pathogenicity, host-agent relations, miscellaneous strains, epidemiology Phylogeny Polymerase Chain Reaction Random Amplified Polymorphic DNA Technique RAPD Serotyping serovar Species Specificity Virology |
| title | Molecular Typing of Bacillus thuringiensis Serovars by RAPD-PCR |
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