Morphology of Molecular Soy Protein Fractions in Binary Composite Gels

• Published in: Langmuir 2009-08, Vol.25 (15), p.8538-8547
• Main Authors: Kasapis, Stefan, Tay, Sok Li
• Format: Article
• Language: English
• Subjects: Electrophoresis, Polyacrylamide Gel; Gels; Gluconates - chemistry; Glycine max - metabolism; Hydrogen-Ion Concentration; Kinetics; Lactones; Macromolecular Substances; Materials: Nano-and Mesostructured Materials, Polymers, Gels, Liquid Crystals, Composites; Microscopy, Electron, Scanning - methods; Molecular Weight; Solvents - chemistry; Soybean Proteins - chemistry; Spectrophotometry - methods; Stress, Mechanical; Temperature; Time Factors
• ISSN: 0743-7463; 1520-5827
• DOI: 10.1021/la803290j

We investigate the structural properties of gels of binary mixtures of the three major soy protein fractions: 11S, 7S, and 2S. Gels are formed at 25 °C in the presence of glucono-δ-lactone and studied using a combination of dynamic rheology and scanning electron microscopy. The rheological data was then modeled using a blending-law approach that yields insights into the solvent distribution between the gelled protein fractions and first-order reaction kinetics that follow the gelation process of the single fractions and their mixtures. Gelled mixtures of 11S and 7S yielded enhanced network strength with increasing solid content; in these gels, 50% more solvent partitioned into the 11S phase as compared to that in the 7S phase. In contrast, the addition of small-molecular-weight counterpart 2S to either 11S or 7S results in a catastrophic drop in the values of the overall strength of the mixture. The unexpected phase behavior has been rationalized on the basis of the high water-holding capacity of 2S; 450% more solvent partitions preferentially into the 2S phase as compared to that in the 11S phase. As the concentration of 2S is increased relative to that of 11S or 7S, it becomes the dominant phase and entraps the polymeric segments of 11S (or 7S), thus preventing them from becoming the structural knots of the gel. In addition to the solvent distribution in the gel, the rates of gelation differ markedly between 11S and 2S (with the 11S rate of gelation being up to 2 orders of magnitude greater); at fixed 11S concentration, the rate of gelation decreases with increasing amounts of 2S, further confirming that the latter essentially becomes the dominant phase in the composite gel.