Distribution and sequence analysis of a novel Ty3‐like element in natural Saccharomyces paradoxus isolates

Little is known about the transposable elements of species closely related to Saccharomyces cerevisiae. We present a novel transposable element in Saccharomyces paradoxus, a close congener of S. cerevisiae. Sequence analysis of this element, designated Ty3‐1p, indicates that it is a homologue of the...

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Bibliographic Details
Published in:Yeast (Chichester, England) England), 2003-07, Vol.20 (9), p.761-770
Main Authors: Fingerman, Ethan G., Dombrowski, Peter G., Francis, Chantal A., Sniegowski, Paul D.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Blotting, Southern
Chromosomes, Fungal - genetics
Cloning, Molecular
DNA Transposable Elements - genetics
DNA, Fungal - chemistry
DNA, Fungal - genetics
Fungal Proteins - genetics
Molecular Sequence Data
Open Reading Frames - genetics
Polymerase Chain Reaction
Saccharomyces - genetics
Saccharomyces cerevisiae
Saccharomyces paradoxus
Sequence Alignment
Sigma Factor - genetics
transposable element evolution
Ty3
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Summary:Little is known about the transposable elements of species closely related to Saccharomyces cerevisiae. We present a novel transposable element in Saccharomyces paradoxus, a close congener of S. cerevisiae. Sequence analysis of this element, designated Ty3‐1p, indicates that it is a homologue of the S. cerevisiae Ty3 element. Ty3‐1p shares 82% nucleotide identity with an S. cerevisiae Ty3 element and appears to be structured identically to Ty3, containing two overlapping open reading frames, six retroviral‐like domains, a J domain, and flanking sigma‐like elements. A sigma element from Ty3‐1p is 75% identical to a Ty3 sigma element. There is no evidence of horizontal transfer of Ty3 in Saccharomyces sensu stricto. We assess the distributions of Ty3p and Ty3 element insertions in natural population samples of S. paradoxus and S. cerevisiae. The S. paradoxus population sample exhibits Ty3p insertions present at a variety of sites at low frequency; this suggests that Ty3p elements are active in the sampled population. The S. cerevisiae population sample exhibits a uniform Ty3 hybridization profile in which all element insertions appear to be fixed. We comment on the possible causes of these contrasting observed distributions (GenBank Accession Nos AY198186 and AY198187). Copyright © 2003 John Wiley & Sons, Ltd.
ISSN:0749-503X
1097-0061
DOI:10.1002/yea.1005